Abstract
We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.
🔬 Techniques
🧪 Sample Preparation
🔬 Cell Lines
🏭 Microscope Brands
🔎 Objectives
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Fig. 1
(Color online) High-speed synthetic aperture phase microscopy setup. Laser: Heu2013Ne laser; GM, dual-axis galvanometer scanning mirror; L1, f = 250 mm lens; BF, backfocal plane of condenser lens; C, ...
Fig. 2
(Color online) Angular images and synthesized images for a live microglia cell. (a), (d) Phase images taken in the image plane with illumination angles of 0u00b0 and 23:3u00b0, respectively. (b) Phase...
Fig. 3
(Color online) Images of a helical cholesterol ribbon [(a), (b)] and a USAF targetlike phase pattern [(c), (d)]. Phase images for angle of 0u00b0 illumination [(a), (c)] and for aperture synthesis [(b...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
0 commentsNo comments yet. Be the first to start a discussion!
Leave a Comment