Abstract
Developing Neurons Make the Cut Neurons in the developing central nervous system of vertebrates derive from cells adjacent to the ventricles that then proliferate and differentiate to populate the brain. As one of these cells begins to differentiate, the cell nucleus migrates toward its new residence, away from the ventricle surface, and the cell stretches out. At some point, like any maturing adolescent, the cell has to leave home. Das and Storey (p. 200 ; see the Perspective by Tozer and Morin ) show that instead of letting go and drawing the trailing process up into the migrating cell, the cell cuts off and discards its first roots. The abscission process leaves behind the primary cilium and any signaling systems localized to the cilium.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
💾 Data Repositories
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Fig. 1
Apical abscission during neuronal differentiation
(A) Time-lapse sequence of a cell undergoing distinct stages of apical abscission, [ Movie S1 ; these are additional frames of a cell shown in supplementary movie 2 in ( 6 )]. ( B-D ) Maximum intensit...
Fig. 2
Apical abscission depends on actin-myosin activity
( A, B ) Time-lapse showing actin localisation (A) ( Movie S8 ) and ( B ) quantification of Actin-TagRFP intensity during apical abscission (average normalised values for 4 cells, error-bars S.E.M.); ...
Fig. 3
Apical abscission dismantles the primary cilium
( A, B ) Time-lapse sequences showing centrosome release into the apical cell-process ( A ; Movie S26 ), while Arl13b-labelled cilium is retained at the apical membrane ( B ; Movie S29 ) . ( C ) Widef...
Fig. 4
N-Cadherin loss promotes apical abscission
( A ) Cells mis-expressing NCad-YFP constrict (white arrowheads) but do not abscise and the centrosome (PACT-tagRFP, magenta arrows) remains at the apical cell-pole ( Movie S35 ). ( B ) N-Cad-YFP mis-...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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