Abstract
AbstractAs the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three‐dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significant advantages to visualize these changes. Structural OCT has been used to investigate the longitudinal development of engineered tissues and cell dynamics such as migration, proliferation, detachment, and cell‐material interactions. Optical techniques that image functional parameters or integrate multiple imaging modalities to provide complementary contrast mechanisms have been developed, such as the integration of optical coherence microscopy with multiphoton microscopy to image structural and functional information from cells in engineered tissue, optical coherence elastography to generate images or maps of strain to reflect the spatially‐dependent biomechanical properties, and spectroscopic OCT to differentiate different cell types. From these results, OCT demonstrates great promise for imaging and visualizing engineered tissues, and the complex cellular dynamics that directly affect their practical and clinical use. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
🏭 Microscope Brands
📷 Detectors
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
Schematic diagram of a time-domain OCT system used in the studies.
Figure 2
Two-dimensional OCT images (a-e), their corresponding histological images (f-j) and three-dimensional OCT images under different stages of engineered tissue development: day 1 (a, f, k, l, and m), day...
Figure 3
Three-dimensional (a-c) and two-dimensional (d-f) OCT images and corresponding histological images (g-i) of cell proliferation in 3-D scaffolds at different stages of development: day 0 (a, d, and g),...
Figure 4
Experimental setup for the integrated optical coherence and multiphoton microscope (OCM/MPM). Abbreviations: BS, beam splitter; CCD, charge-coupled line-scan camera; CU, collimating unit; DM, dichroic...
Figure 5
Integrated OCM/MPM imaging of GFP-vinculin fibroblasts seeded on a microtextured substrate under static (a-c) and dynamic (d-f) culture. (a) and (d) OCM images showing both cells and substrate. (b) an...
Figure 6
Three-dimensional OCM/MPM images of fibroblasts from a transgenic GFP mouse cultured in a Matrigel scaffold and GFP-vinculin fibroblasts seeded in a 3-D Matrigel scaffold cultured under static (a and ...
Figure 7
Optical coherence elastography of developing engineered tissue. Structural OCT images (a, e, i, and m), displacement maps (b, f, j, and n), strain maps (c, g, k, and o) and representative histological...
Figure 8
SOCT images of two types of cells in engineered tissues. (a) Structural OCT image of fibroblasts in a 3-D scaffold. (b) SOCT analysis of (a). (c) Structural OCT image of macrophages in a 3-D scaffold....
Figure 9
In vivo OCM (a-c) and MPM (d-f) images of epidermal and dermal human skin layers at depths of 25 u03bcm (a and d), 50 u03bcm (b and e), and 70 u03bcm (c and f). The circular structures are dermal papi...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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