Abstract
Multicolour, three-dimensional stochastic optical reconstruction microscopy now makes it possible to image cellular structures with near molecular-scale resolution.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
💾 Data Repositories
🏛️ Research Organizations (ROR)
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📊 Figures
Fig. 1
The principle of STORM
For a densely labeled cellular structure shown in a , only a sparse set of fluorescent labels are activated at any given time such that their images (green circles) do not overlap ( b ). b , The posit...
Fig. 2
STORM imaging of cells with photo-switchable dyes and fluorescent proteins
a , STORM image of microtubules in a BS-C-1 cell. The microtubules are immunostained with antibodies that are labeled with photo-switchable Alexa Fluor 647. b and c , Conventional (b) and STORM (c) im...
Fig. 3
Multicolour STORM
The microtubules and clathrin-coated pits in a BS-C-1 cell are immunostained with Cy2-Alexa 647-labeled and Cy3-Alexa 647-labeled antibodies, respectively. a , Conventional fluorescence image. b , STO...
Figure 4
Three-dimensional STORM
a , Conventional image of clathrin-coated pits in a BS-C-1 cell. Clathrin is immuno-stained with Cy3-Alexa 647-labeled antibodies. b , x-y cross-section of the corresponding 3D STORM image. c , Serial...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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