Abstract
We report a mitochondria-specific glutathione (GSH) probe-designated as Mito-RealThiol (MitoRT)-that can monitor in vivo real-time mitochondrial glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.
🔬 Techniques
✨ Fluorophores
🔬 Cell Lines
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
The reversible Michael addition reaction between MitoRT and GSH. The function of each moiety in RT is highlighted (QY: quantum yield). The wiggled bond indicates either cis or trans configuration of t...
Figure 2
Characterization of reversible reaction-based mitochondria-specific glutathione probe MitoRT. (a) UV-vis (unshaded) and fluorescence (shaded) spectra of MitoRT (green) and MitoRT-GSH (blue), the GSH a...
Figure 3
MitoRT-based imaging of mitochondrial glutathione in living cells. (a) Confocal and ratiometric images of HeLa cells stained with MitoRT and MitoTracker. MitoRT and MitoRT-GSH are well colocalized wit...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
0 commentsNo comments yet. Be the first to start a discussion!
Leave a Comment