Abstract
Genetically encodable fluorescent biosensors provide spatiotemporal information on their target analytes in a label-free manner, which has enabled the study of cell biology and signaling in living cells. Over the past three decades, fueled by the development of a wide palette of fluorescent proteins, protein-based fluorescent biosensors against a broad array of targets have been developed. Recently, with the development of fluorogenic RNA aptamer-dye pairs that function in live cells, RNA-based fluorescent (RBF) biosensors have emerged as a complementary class of biosensors. Here we review the current state-of-the-art for fluorogenic RNA aptamers and RBF biosensors for imaging small molecules and RNAs, and highlight some emerging opportunities.
🔬 Techniques
🧬 Organisms
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
🧪 Reagent Suppliers
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1.
Fluorogenic RNA aptamers-dye pairs. Above, schematic representations of two types of fluorogenic RNA aptamers based on their paired dyes: single dyes (A) and fluorophore-quencher conjugates (B). Yello...
Figure 2.
Design strategies of RNA-based fluorescent biosensors for molecular sensing. Functional RBF biosensors can be generated either by (A) splitting the dye-binding aptamer [ 16 ] or (B) splitting the liga...
Figure 3.
Examples of RBF biosensors for RNA sensing. (A) Split-binding domain strategy was applied on Spinach and SRB-2 aptamer to generate biosensors for miRNA [ 52 u2022, 27 u2022]. (B) Destabilizing the flu...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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