🏆 Foundational Paper

Cell-Permeant Large Stokes Shift Dyes for Transfection-Free Multicolor Nanoscopy.

Butkevich Alexey N, Lukinavičius Gražvydas, D'Este Elisa, Hell Stefan W

📰 Journal of the American Chemical Society 📅 2017 📊 123 citations
DOI PubMed

Abstract

We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength.

🔬 Techniques

Stimulated Emission Depletion Microscopy Super-Resolution Microscopy

🧪 Sample Preparation

Transfection

🔬 Cell Lines

HeLa (Henrietta Lacks) Human Osteosarcoma U2OS Primary Fibroblasts

🏛️ Research Organizations (ROR)

Affiliated research institutions:

🏛 Max Planck Society 🏛 Max Planck Institute for Biophysical Chemistry 🏛 Max Planck Institute of Biophysics

🏛️ Imaging Facility

🏛️ Max Planck Institute

💬 Discussion

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