Live cell imaging of the HIV-1 life cycle.
Technology developed in the past 10 years has dramatically increased the ability of researchers to directly visualize and measure various stages of the HIV type...
Technique • 2561 papers
Technology developed in the past 10 years has dramatically increased the ability of researchers to directly visualize and measure various stages of the HIV type...
BACKGROUND: In plants, a complex cell wall protects cells and defines their shape. Cellulose fibrils form a multilayered network inside the cell-wall matrix that plays...
ABSTRACT Epstein–Barr virus (EBV) infection is nearly ubiquitous and strongly linked to multiple sclerosis (MS), but how EBV-infected B cells communicate with distal tissues remains...
Expansion microscopy (ExM) is a powerful technique that enhances optical resolution by physically enlarging biological samples. The enlargement increases the distances between fluorophores and makes...
This chapter presents a step-by-step protocol to resolve the organization of the nuclear pore complex (NPC) in HeLa-NUP107-GFP cells by eight-fold expansion microscopy (ExM). To...
Bioorthogonal labelling of living cells enables the incorporation of small, chemically inert units (alkynes or azides) into nascent chains of biomolecules allowing the tracking of...
Abstract Nanoscale fluorescence imaging with a large-field view is invaluable for many applications such as imaging of subcellular structures, visualizing protein interaction, and high-resolution tissue...
Abstract Cell cycle progression is tightly controlled by the regulated synthesis and degradation of Cyclins, such as Cyclin A and Cyclin B, which activate CDK1...
One-Sentence Summary Raf1 is present within the mitochondrial matrix, where it binds GLS to regulate glutamine catabolism and tumorigenesis. In cancer, Raf1 activation occurs via...
Abstract From molecules to organelles, cells exhibit recurring structural motifs across multiple scales. Understanding these structures provides insights into their functional roles. While super-resolution microscopy...