🏆 Foundational Paper

Hybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging.

Rao Bin, Li Li, Maslov Konstantin, Wang Lihong

📰 Optics letters 📅 2010 📊 105 citations
DOI PubMed

Abstract

Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facilitating many demanding biomedical applications. With a high-pulse-repetition-rate laser, the hybrid-scanning OR-PAM can acquire one-dimensional depth-resolved images (A-lines) at 5 kHz and two-dimensional B-scan images containing 800 A-lines at 6.25 Hz. We demonstrated in vivo in a mouse three-dimensional imaging of the iris vasculature in 128 s for an 800x800x200 data set and of the ear vasculature in 256 s for an 800x1600x200 data set.

🔬 Techniques

Confocal Laser Scanning Microscopy Multiphoton Microscopy Optical Coherence Tomography Photoacoustic Microscopy

💻 Software

ScanImage

✨ Fluorophores

DiD

🧪 Sample Preparation

Transduction

🏭 Microscope Brands

Thorlabs

💻 Software Details

Image Acquisition:
ScanImage

🏛️ Research Organizations (ROR)

Affiliated research institutions:

🏛 Washington University in St. Louis 🏛 Optica

📊 Figures

Fig. 1

Fig. 1

Schematic of the hybrid-scanning optical-resolution photoacoustic microscopy system.

Fig. 2

Fig. 2

(A) Light microscopy image and (B) maximum amplitude projection PA image of the 6 th and 7 th groups in a USAF-1951 resolution target acquired by the hybrid-scanning optical-resolution photoacoustic m...

Fig. 3

Fig. 3

(A) B-mode PA image (1.5 mm u00d7 4.0 mm) scanning on the back-side of an alignment plate (LCPA1, Thorlabs). (B) Peak PA signal amplitude versus depth.

Fig. 4

Fig. 4

Maximum amplitude projection PA images of the iris vasculature in a mouse (A) under dim ambient illumination condition and (C) bright ambient light illumination condition. Both the major artery circle...

Fig. 5

Fig. 5

(A) Photograph of a live mouse ear. (B) Maximum amplitude projection PA image of the mouse ear vasculature acquired in vivo.

Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.

🏛️ Imaging Facility

🏛️ Washington University

💬 Discussion

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