Abstract
The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules.
🔬 Techniques
🧬 Organisms
💻 Software
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
📷 Detectors
💻 Software Details
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
Generalized microscope configurations for single-molecule imaging in living cells.
Figure 2
Some general approaches to SMS in live cells.
Figure 3
Single molecules in cell membranes. (A) Cell-penetrating peptides labeled with a DCDHF organic fluorophore in the plasma membrane of mammalian cells. At low enough concentrations of labeled peptides, ...
Figure 4
Single molecules in bacteria. (A) FP-labeled MreB, an actin homolog, shows treadmilling through short MreB filaments in a living C. crescentus cell. Directional motion of MreBu2013FP was measured by i...
Figure 5
First live-cell super-resolution SMS experiments. (A) Clusters of hemagglutinin in the membrane of a living fibroblast cell. The right frame is a zoomed-in portion of the left image. The jagged border...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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