Technique • 1734 papers
Abstract The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits, which is of fundamental...
The neural basis of abnormal social behavior in autism spectrum disorders (ASDs) remains incompletely understood. Here we used two complementary but independent brain-wide mapping approaches,...
The visualization of whole organs and organisms through tissue clearing and fluorescence volumetric imaging has revolutionized the way we look at biological samples. Its application to...
AbstractStructural elucidation and molecular scrutiny of cerebral vasculature is crucial for understanding the functions and diseases of the brain. Here, we introduce SeeNet, a method...
Conventional immunoassays such as ELISA are widely used in serological testing, yet their reliance on multi-step workflows and labeled reagents limits diagnostic scalability and speed....
Abstract Understanding how neurons integrate synaptic inputs requires imaging techniques capable of capturing rapid, three-dimensional dendritic events. These processes occur on millisecond timescales and submicron...
Abstract Background How does neuronal activity change as an animal transitions from being awake to a state of general anesthesia? Previous studies used C. elegans...
Expansion microscopy (ExM) is a powerful technique that enhances optical resolution by physically enlarging biological samples. The enlargement increases the distances between fluorophores and makes...
This chapter presents a step-by-step protocol to resolve the organization of the nuclear pore complex (NPC) in HeLa-NUP107-GFP cells by eight-fold expansion microscopy (ExM). To...
Organic solvent-based tissue clearing methods are widely used for whole-brain imaging but often compromise endogenous fluorescence. Existing protocols, such as iDISCO and fluorescence-preserving variants, have...