Abstract
Fundamental to the success of cell and developmental biology is the ability to tease apart molecular organization in cells and tissues by localizing specific proteins with respect to one another in a native cellular context. However, many key cellular structures (from mitochondrial cristae to nuclear pores) lie below the diffraction limit of visible light, precluding analysis of their organization by conventional approaches. Point-localization superresolution microscopy techniques, such as PALM and STORM, are poised to resolve, with unprecedented clarity, the organizational principles of macromolecular complexes within cells, thus leading to deeper insights into cellular function in both health and disease.
🔬 Techniques
🧬 Organisms
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
Principle of photoactivation/switching based pointillist superresolution microscopy. (A) The probe molecules are in the dark state and are invisible at the start of the experiment. A sparse subset of ...
Figure 2
Working principle of bleaching/blinking based superresolution microscopy. Subtracting consecutive image frames from each other isolates single fluorescent spots, corresponding to single molecules that...
Figure 3
Exhibition of the macromolecular details reconstructed using point-localization microscopy methods. (A) Class averaged dSTORM reconstruction of components of the nuclear pore complex (NPC) from isolat...
Figure 4
(Au2013B) Pair-correlation PALM (PC-PALM) analysis enables the estimation of physical parameters of molecular clusters in single molecule super-resolution images. (A) Super-resolution PALM image of Ly...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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