Abstract
Quantifying the amount and defining the location of metal ions in cells and organisms are critical steps in understanding metal homeostasis and how dyshomeostasis causes or is a consequence of disease. A number of recent advances have been made in the development and application of analytical methods to visualize metal ions in biological specimens. Here, we briefly summarize these advances before focusing in more depth on probes for examining transition metals in living cells with high spatial and temporal resolution using fluorescence microscopy. This article is part of a Special Issue entitled: Cell Biology of Metals.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
🏭 Microscope Brands
🧪 Reagent Suppliers
💾 Data Repositories
🏛️ Research Organizations (ROR)
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📊 Figures
Figure 1
General Mechanisms of Live-Cell Metal Sensing. (A) Binding of zinc by a multi-FP sensor changes the conformation and/or distance between the donor fluorophore (CFP) and the acceptor fluorophore (YFP),...
Figure 2
Subcellular localization of metal sensors for detecting labile zinc (green), copper (blue) and iron (red) in live cells. Zinc sensor RhodZin-3 [ 75 ], mito-ZifCY1 [ 48 ], mito-ZP1 [ 56 ] and copper se...
Figure 3
Summary of major intensity-based small molecule sensors for Zn 2+ (cross), Cu 1+ (grey circle), and Cu 2+ (black circle). The figure is intended to present dissociation constant (pK D , y-axis), and a...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
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