Technique ā¢ 1315 papers
Extensive studies have shown that cells can sense and modulate the biomechanical properties of the ECM within their resident microenvironment. Thus, targeting the mechanotransduction signaling...
AbstractWe present singleāmolecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and singleāmolecule fluorescence experiments proved mCherry...
SIGNIFICANCE: The noninvasive assessment of oxidative stress in the retinal pigment epithelium (RPE), a key factor in the pathogenesis of age-related macular degeneration (AMD), is...
Photonic imaging technologies have profoundly transformed neuro-ophthalmic diagnostics by enabling non-invasive visualization of neurodegenerative processes at the retinal level. This review examines how advanced light-based...
Fluorescence lifetime imaging microscopy (FLIM) has proven to be a useful method for analyzing various aspects of material science and biology, like the supramolecular organization...
Structural changes in dendritic spines underlie long-term potentiation (LTP). While CaMKII has been considered as the primary driver of these changes, we show that transient,...
SIGNIFICANCE: Prostate biopsy remains the gold standard for prostate cancer (PCa) diagnosis and treatment planning. However, current techniques suffer from low cancer detection rates, with...
ABSTRACT Conventional analytical methods for fluorescence lifetime imaging (FLI) require the instrument response function (IRF) alongside temporal fluorescence decay to accurately estimate lifetime parameters. While...
Abstract Significance Standard methods to characterize peripheral blood mononuclear cells (PBMCs) are often destructive, lack metabolic information, or do not provide single-cell resolution. Label-free tools...
Fluorescence lifetime imaging microscopy (FLIM) measures the average time that a fluorophore spends in the excited state. FLIM is exceptionally useful as an imaging tool...