Stellaris

Stellaris

Microscope • 18 papers

⭐ Foundational

Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues.

Current protocols in cell biology 2018 📊 212

Expansion microscopy (ExM) is a recently developed technique that enables nanoscale-resolution imaging of preserved cells and tissues on conventional diffraction-limited microscopes via isotropic physical expansion...

3D ImagingConfocal Laser Scanning MicroscopyExpansion MicroscopyLight Sheet Fluorescence MicroscopyOptical SectioningSpinning Disk Confocal MicroscopySP8StellarisZeiss Lightsheet Z.1 Light SheetArivisImarisGFPYFPCell CultureExpansion MicroscopyFixationFluorescence In Situ HybridizationGlutaraldehydeImmunostainingIn Situ HybridizationParaformaldehyde FixationPermeabilizationRNAscopeSingle-Molecule Fluorescence In Situ HybridizationTissue ClearingVibratomeEmbryonic Stem CellHeLa (Henrietta Lacks)Zeiss Lightsheet Z.1 Light SheetArivisImarisBroad InstituteJanelia Research CampusMassachusetts Institute of TechnologyMITMatTekSigma-AldrichCurrent Protocols
⭐ Foundational

Nanoscale imaging of RNA with expansion microscopy.

Nature methods 2016 📊 373

The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in...

Confocal Laser Scanning MicroscopyEpifluorescence MicroscopyExpansion MicroscopyLight Sheet Fluorescence MicroscopyOptical SectioningSpinning Disk Confocal MicroscopyCSU-X1Nikon TiSP8StellarisTiYokogawa CSU-X1 Spinning DiskZeiss Lightsheet Z.1 Light SheetNIS-ElementsZENATTO 647NCy5EdUFITCGFPQuasArYFPCell CultureExpansion MicroscopyFixationFluorescence In Situ HybridizationIn Situ HybridizationSingle-Molecule Fluorescence In Situ HybridizationVibratomeHeLa (Henrietta Lacks)Human Embryonic Kidney 293Yokogawa CSU-X1 Spinning DiskZeiss Lightsheet Z.1 Light Sheetarivis Proarivis Vision4DFijiImarisNIS-ElementsZENMATLABHarvard Medical SchoolMassachusetts Institute of TechnologyUniversity of PennsylvaniaWyss Institute for Biologically Inspired EngineeringInvitrogenLife TechnologiesNew England BiolabsAndor sCMOSPCO sCMOSPCO.Edge 5.sCMOS40x/1.15 NA water4x/0.2 NA air60x/1.4 NA oil525/40 bandpass607/36 bandpass685/40 bandpass
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⭐ Foundational

smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability.

Nucleic acids research 2016 📊 388

Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. We present smiFISH (single molecule inexpensive FISH), an...

Widefield Fluorescence MicroscopySP8StellarisMetaMorphATTO 565CellMaskCy3Cy5DAPIGFPExpansion MicroscopySingle-Molecule Fluorescence In Situ HybridizationEmbryonic Stem CellHeLa (Henrietta Lacks)SP8StellarisCellProfilerImarisMetaMorphMATLABCNRSÉcole Nationale Supérieure des Mines de ParisINSERMInstitut CurieInstitut PasteurLebanese UniversityResearch UniversityUniversité de MontpellierThermo Fisher ScientificHamamatsu Orca Flash 4.0Lumencor sCMOSMolecular Devices CCDNikon 60x/1.4 NAZeiss 100x/1.4 NA
💾 Data

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