Technique • 1207 papers
Chromosome segregation during mitosis and meiosis relies on the spindle and the functions of numerous microtubule-associated proteins (MAPs). One of the best-studied spindle MAPs is...
New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes...
Abstract Coordinated cortical activity underlies voluntary movement planning, yet its disruption is implicated in neurodevelopmental disorders like autism spectrum disorder (ASD). Mutations in the SHANK3...
Studying the function of healthy corneal endothelial cells has been challenging due to a variety of culturing strategies. Here, we present a standardized protocol for...
Quantifying cell-induced material deformation provides useful information concerning how cells sense and respond to the physical properties of their microenvironment. While many approaches exist for...
SIGNIFICANCE: High-throughput live-cell imaging is crucial for biological applications, including organ-on-a-chip (OoaC) platforms, yet conventional optical systems face a fundamental trade-off between magnification and field...
Confocal microscopy offers important advantages compared to conventional epifluorescence microscopy. It works as an "optical microtome" leading to a accurate image resolution of a defined...
Performing reflection-mode (backscatter-mode) confocal microscopy on cells growing on reflective substrates gives images that have improved contrast and are more easily interpreted than standard reflection-mode...
Abstract Oligonucleotide-carbohydrate conjugates is an efficient strategy to provide cell-specific delivery of oligonucleotides. Combining the multivalent nature of molecular spherical nucleic acids (MSNAs) with a...