Technique • 1301 papers
COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast...
Quorum sensing is a chemical signaling mechanism used by bacteria to communicate and orchestrate group behaviors. Multiple feedback loops exist in the quorum-sensing circuit of...
The design and optimization of fluorescent molecules has driven the ability to interrogate complex biological events in real time. Notably, most advances in bioimaging fluorophores...
Abstract In this work, we present a proof‐of‐principle experiment that extends advanced live cell microscopy to the scale of pool‐generated strain libraries. We achieve this...
BACKGROUND: The poor prognosis for glioblastoma patients is caused by the diffuse infiltrative growth pattern of the tumor. Therefore, the molecular and cellular processes underlying...
The heterozygous NLGF mouse is considered a mild model of Alzheimer’s disease (AD), closely reflecting the gradual progression observed in humans, compared to more aggressive...
Electrical activity and neurotransmitter release are tightly coupled in neurons, but co-registered measurements of both modalities from the same subcellular site in real time have...
Kainate receptors (KARs) are critical mediators and modulators of synaptic transmission which undergo rapid activation and desensitization upon binding of the neurotransmitter glutamate. Under various...
Expansion microscopy (ExM) is a powerful technique that enhances optical resolution by physically enlarging biological samples. The enlargement increases the distances between fluorophores and makes...
This chapter presents a step-by-step protocol to resolve the organization of the nuclear pore complex (NPC) in HeLa-NUP107-GFP cells by eight-fold expansion microscopy (ExM). To...