Abstract
Metabolic labeling of azido sugars combined with two-photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both cell culture and intact human tissue slices.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
🏭 Microscope Brands
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
A technique for imaging the glycosylation state of glycoproteins through the use of metabolic labeling and 2-photon fluorescence lifetime imaging microscopy (FLIM). Cells or tissues are fed the azido ...
Figure 2
Incorporation of SiaNAz into integrin u03b1 V u03b2 3 and U87MG cell surface glycoconjugates. (A) Lectin blot of u03b1 and u03b2 subunits of immunoprecipitated u03b1 V u03b2 3 with u03b12,6 (SNA) and ...
Figure 3
FLIM imaging of sialylated integrin u03b1 V u03b2 3 . 2-Photon FLIM images (A,B) of U87MG cells treated with either Ac 4 ManNAc (A) or Ac 4 ManNAz (B), followed by DIBAC-647 and Fab-594. FLIM images a...
Figure 4
FLIM FRET is dependent on the proximity of the acceptor dye to Fab-594 and the target proteinu2019s glycosylation state. FLIM images of (A) Ac 4 ManNAz-fed U87MG cells treated with DIBAC-647 alone, (B...
Figure 5
FLIM images and lifetime histogram of grade 3u20134 human prostate adenocarinoma tissue slices. Tissue slices were incubated with either Ac 4 ManNAc (A), Ac 4 ManNAz (B), or Ac 4 ManNAz and sialidase ...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
0 commentsNo comments yet. Be the first to start a discussion!
Leave a Comment