Abstract
We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
🏭 Microscope Brands
🔴 Lasers
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Fig. 1
a, Portable two-photon microscope. Electrical lines control the MEMS scanner and focusing micromotor. b, The scanner die is wirebonded onto electrodes on the printed circuit board (PCB). c, Computer-a...
Fig. 2
(Color online) a, Optical deflection angle versus dc voltage, and b, frequency response function for inner (blue solid curve) and outer (red dashed curve) axes of a 1 mm u00d71 mm MEMS scanner. For ea...
Fig. 3
au2013c, Images of neocortical capillaries, averaged over eight frames acquired over 2 s at 4 Hz. du2013f, Line images taken by driving only the scanneru2019s outer axis, at its resonant frequency (56...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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