Technique • 129 papers
Metabolism is highly dynamic and intrinsically heterogeneous at the cellular level. Although fluorescence microscopy has been commonly used for single cell analysis, bulky fluorescent probes...
Intraductal carcinoma of the prostate (IDC-P) is a very aggressive histopathological subtype of prostate cancer (PCa) that is strongly associated with poor clinical outcomes but...
Mapping the localization of multiple proteins in their native three-dimensional (3D) context would be useful across many areas of biomedicine, but multiplexed fluorescence imaging has...
Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests...
Advanced optical methods combined with various probes pave the way toward molecular imaging within living cells. However, major challenges are associated with the need to...
Optical microscopy has generated great impact for modern research. While fluorescence microscopy provides the ultimate sensitivity, it generally lacks chemical information. Complementarily, vibrational imaging methods...
Three-dimensional visualization of tissue structures using optical microscopy facilitates the understanding of biological functions. However, optical microscopy is limited in tissue penetration due to severe...
AbstractImaging of nucleic acids is important for studying cellular processes such as cell division and apoptosis. A noninvasive label‐free technique is attractive. Raman spectroscopy provides...
Metabolic fingerprinting provides valuable information on the physiopathological states of cells and tissues. Traditional imaging mass spectrometry and magnetic resonance imaging are unable to probe...
Optical multiplexing has a large impact in photonics, the life sciences and biomedicine. However, current technology is limited by a 'multiplexing ceiling' from existing optical...