Abstract
Optical imaging has revolutionized our ability to monitor brain activity, spanning spatial scales from synapses to cells to circuits. Here, we summarize the rapid development and application of mesoscopic imaging, a widefield fluorescence-based approach that balances high spatiotemporal resolution with extraordinarily large fields of view. By leveraging the continued expansion of fluorescent reporters for neuronal activity and novel strategies for indicator expression, mesoscopic analysis enables measurement and correlation of network dynamics with behavioral state and task performance. Moreover, the combination of widefield imaging with cellular resolution methods such as two-photon microscopy and electrophysiology is bridging boundaries between cellular and network analyses. Overall, mesoscopic imaging provides a powerful option in the optical toolbox for investigation of brain function.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
🏭 Microscope Brands
🧪 Reagent Suppliers
📷 Detectors
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1.
Mesoscopic imaging of neural activity in the mouse neocortex.
A , Schematic illustrating the imaging system for acquiring mesoscopic fluorescence imaging data in the mouse. Ca2+ indicators are excited via output from an LED engine (violet and blue), and emitted ...
Figure 2.
Two examples for parcellating (segmenting) mesoscopic imaging data collected from a single mouse, showing the averaged response to a visual stimulus (arrow). Using a standard atlas based on averaged a...
Figure 3.
Example showing analysis of functional connectivity in cortical networks determined by dual 2-photon and mesoscopic imaging. A , Example image frames showing raw mesoscopic (upper) and 2-photon (lower...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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