Seronegative Full-house Nephropathy with Crohn’s Disease.
Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disease. Lupus nephritis (LN) is a major risk factor for mortality in SLE, and glomerular "full-house"...
Immunofluorescence
Immunofluorescence
Technique • 24 papers
A modified immunofluorescence in situ hybridization method to detect long non-coding RNAs and proteins in frozen spinal cord sections.
Immunofluorescence in situ hybridization (immuno-FISH) is widely used to co-detect RNAs and proteins in order to study their spatial distribution in cells. The present study...
Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae.
AbstractObjectiveThe objective was to investigate fixative solutions: 3.7% formaldehyde, 4% paraformaldehyde, 4% paraformaldehyde in the cytoskeletal buffer and 4% paraformaldehyde in PHEM buffer (containing PIPES,...
Determining membrane protein topologies in single cells and high-throughput screening applications.
AbstractCorrect localization and topology are crucial for a protein's cellular function. To determine topologies of membrane proteins, a new technique, called fluorescence protease protection (FPP)...
Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy.
INTRODUCTION: While protective plasma cells (PCs) are an important part of the individual's immune defense, autoreactive plasma cells such as dsDNA-specific plasma cells contribute to...
Properties of Uncommon Indirect Immunofluorescence Staining Patterns Determined during Antinuclear Antibody Detection on HEp-2 Cells.
In this study, we aimed to assess the prevalence of uncommon staining patterns found during testing for the presence of antinuclear antibodies (ANA) and to...
A live-cell imaging system for visualizing the transport of Marburg virus nucleocapsid-like structures.
Abstract Background Live-cell imaging is a powerful tool for visualization of the spatio-temporal dynamics of moving signals in living cells. Although this technique can be...
💾 Data
In-House Immunofluorescence Assay for Detection of SARS-CoV-2 Antigens in Cells from Nasopharyngeal Swabs as a Diagnostic Method for COVID-19.
Immunofluorescence is a traditional diagnostic method for respiratory viruses, allowing rapid, simple and accurate diagnosis, with specific benefits of direct visualization of antigens-of-interest and quality...
BRAF Inhibitor Resistance Confers Increased Sensitivity to Mitotic Inhibitors.
Single agent and combination therapy with BRAF V600E/K and MEK inhibitors have remarkable efficacy against melanoma tumors with activating BRAF mutations, but in most cases...
💾 Data
🏷️ 8 RRIDs
A quantitative comparison of antibodies against epitope tags for immunofluorescence detection.
Epitope tags recognized by specific antibodies have been widely used over the last few decades, notably to localize tagged proteins within cells by immunofluorescence. The...