dichroic 450

dichroic 450

Filter • 3 papers

Verifying the function and localization of genetically encoded Ca2+ sensors and converting FRET ratios to Ca2+ concentrations.

Cold Spring Harbor protocols 2015 📊 4

Genetically encoded, ratiometric, fluorescent Ca(2+) biosensors can be used in living cells to quantitatively measure free Ca(2+) concentrations in the cytosol or in organelles. This...

Confocal Laser Scanning MicroscopyDifferential Interference Contrast MicroscopyEpifluorescence MicroscopyFluorescence MicroscopyFörster Resonance Energy TransferWidefield Fluorescence MicroscopyNIS-ElementsCFPmCherryMitoTrackerYFPCell CultureLipofectionPermeabilizationTransfectionHeLa (Henrietta Lacks)ImageJNIS-ElementsExcelMATLABUniversity of ColoradoCorningLife TechnologiesCold Spring Harbor ProtocolsCCDdichroic 450dichroic 515

Quantitative measurement of Ca2+ and Zn2+ in mammalian cells using genetically encoded fluorescent biosensors.

Methods in molecular biology (Clifton, N.J.) 2014 📊 31

Genetically encoded, ratiometric, fluorescent biosensors can be used to quantitatively measure intracellular ion concentrations in living cells. We describe important factors to consider when selecting...

Confocal Laser Scanning MicroscopyDifferential Interference Contrast MicroscopyEpifluorescence MicroscopyFörster Resonance Energy TransferImmunofluorescence MicroscopyMicroManagerNIS-ElementsCFPMitoTrackerYFPCell CultureFixationImmunofluorescenceLipofectionPermeabilizationTransfectionHeLa (Henrietta Lacks)ImageJMicroManagerNIS-ElementsExcelMATLABUniversity of Colorado BoulderCorningLife TechnologiesMerckSigma-AldrichMethods in Molecular BiologyCCDdichroic 450dichroic 515
⭐ Foundational

Measuring calcium dynamics in living cells with genetically encodable calcium indicators.

Methods (San Diego, Calif.) 2008 📊 125

Genetically encoded calcium indicators (GECIs) allow researchers to measure calcium dynamics in specific targeted locations within living cells. Such indicators enable dissection of the spatial...

Calcium ImagingFluorescence MicroscopyAxiovertZeiss AxiovertExcelFijiImageJCFPCitrineEYFPVenusYFPCell CultureElectroporationLipofectionTransductionTransfectionAxiovertImageJExcelUniversity of ColoradoInvitrogenPhotometrics CCDPhotometrics CoolSnapdichroic 450dichroic 515

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