Abstract
High-throughput imaging (HTI) is a powerful tool in the discovery of cellular disease mechanisms. While traditional approaches to identify disease pathways often rely on knowledge of the causative genetic defect, HTI-based screens offer an unbiased discovery approach based on any morphological or functional defects of disease cells or tissues. In this review, we provide an overview of the use of HTI for the study of human disease mechanisms. We discuss key technical aspects of HTI and highlight representative examples of its practical applications for the discovery of molecular mechanisms of disease, focusing on infectious diseases and host-pathogen interactions, cancer, and rare genetic diseases. We also present some of the current challenges and possible solutions offered by novel cell culture systems and genome engineering approaches.
🔬 Techniques
🧬 Organisms
🧪 Sample Preparation
🔬 Cell Lines
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Fig. 1
Key Figure. Principles of High-Throughput Imaging (HTI)
HTI is a sequential workflow of automated operations. Cells are treated in a multi-well format with perturbing agents (small molecules, siRNA, CRISPR/Cas9 reagents (crRNA:trcrRNA), plasmid vectors) to...
Fig. 2
A) Automated Microscopy
Automated microscopes can be programmed to acquire images in all wells at randomly pre-selected positions (fields of view) in each well, in multiple spectral channels to visualize different fluorescen...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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