Abstract
Technology developed in the past 10 years has dramatically increased the ability of researchers to directly visualize and measure various stages of the HIV type 1 (HIV-1) life cycle. In many cases, imaging-based approaches have filled critical gaps in our understanding of how certain aspects of viral replication occur in cells. Specifically, live cell imaging has allowed a better understanding of dynamic, transient events that occur during HIV-1 replication, including the steps involved in viral fusion, trafficking of the viral nucleoprotein complex in the cytoplasm and even the nucleus during infection and the formation of new virions from an infected cell. In this review, we discuss how researchers have exploited fluorescent microscopy methodologies to observe and quantify these events occurring during the replication of HIV-1 in living cells.
🔬 Techniques
✨ Fluorophores
🧪 Sample Preparation
🔬 Cell Lines
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
Trafficking of HIV-1 viral complexes on microtubules. In this time lapse series, human osteosarcoma cells were microinjected with fluorescently labeled tubulin protein and infected with VSV-g pseudoty...
Figure 2
Visualizing the cell-to-cell contacts that mediate viral transfer. (a) The virological synapse visualized by Jolly and coworkers [ 47 ]. Cell-to-cell contact mediates the recruitment of CD4 receptor (...
Figure I
The power of the OMX imaging system. (a) Polystyrene fluorescent beads approximately the size of virions (0.100 u03bcm) were allowed to puddle on a #1.5 coverslip before imaging on a deconvolution flu...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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