Live cell imaging of the HIV-1 life cycle.
Technology developed in the past 10 years has dramatically increased the ability of researchers to directly visualize and measure various stages of the HIV type...
Correlative Light and Electron Microscopy
Correlative Light and Electron Microscopy
Technique • 298 papers
RNA-based CLEM (RCLEM) bridging RNA localisation and ultrastructural mapping in 3D.
Abstract Spatial transcriptomics and in situ hybridisation techniques have become essential for contextualising single‐cell RNA sequencing (scRNA‐seq) data by mapping cell type– and state–specific mRNA...
Cryo-FIB-enabled 3D imaging of biological specimens.
Abstract Cryo-electron tomography (cryo-ET) has become a key technique for observing the three-dimensional structures of biomolecular complexes and organelles in cellular and tissue environments in...
Volume electron microscopy in axon regeneration research: insights into mitochondria, endoplasmic reticulum, and membrane contacts.
Analysing organelle structures in tissue samples that preserve the in vivo environment, rather than in isolated cells, can provide valuable information on the mechanisms underlying...
Cryo-electron tomography reveals paracellular claudin-15 pores at the tight junction.
ABSTRACT Tight junctions (TJs) are composed of anastomosing strands between epithelial cells. Members of the claudin family of proteins reside within TJ strands and either...
Digging Deep into Alzheimer Disease: How Electron Microscopy Helps Elucidating Its Pathogenesis.
Abstract Alzheimer disease (AD), first described by Alzheimer and Perusini in the early twentieth century, is a devastating neurodegenerative disorder without a definitive cure. Unraveling...
Cryo-electron tomography reveals paracellular claudin-15 pores at the tight junction.
Tight junctions (TJs) within simple epithelia are composed of networks of anastomosing strands at the apical end of the intercellular space. Members of the claudin...
Reviving formalin-fixed, paraffin embedded (FFPE) tissues for on-slide and multiscale correlative microscopy.
Correlative microscopy integrates complementary imaging modalities to enable structural analysis of biological samples across multiple length scales. Here, we present two correlative workflows for formalin-fixed,...
Structural dynamics of α-Synuclein: Multi-scale imaging insights into pathological progression across Synucleinopathies.
The misfolding and aberrant aggregation of alpha-synuclein (α-syn) constitute the central pathological hallmark of a spectrum of synucleinopathies, including Parkinson's disease, dementia with Lewy bodies,...
Dysregulation of a novel autophagosome-mitochondria contact contributes to tauopathy-related neurodegeneration by disrupting autophagy.
Beyond their role in energy production, mitochondria also interact with other organelles through forming membrane contacts that serve as central hubs of cellular metabolism and...