Abstract
Quantum dots are a powerful fluorophore family with desirable attributes for fluorescence imaging. They have been used in several animal models with direct clinical relevance, including sentinel lymph node mapping, tracing vasculature and lymphatics, and targeting specific lesions for diagnosis and removal. (1-12) Despite significant interest for use in translational applications, little is known about the persistence and long-term fate of quantum dots in vivo. We have observed fluorescence of quantum dots injected into Balb/c and nude mice for up to two-years post injection using both whole-body and microscopic fluorescence techniques. Two-photon spectral microscopy was used to verify the existence of quantum dots within two-year tissues, but also revealed a range of significantly blue-shifted emission peaks with increased bandwidths. Systemically administered quantum dots persist and retain fluorescence for up to two-years in vivo, but with significantly blue-shifted emission.
🔬 Techniques
🔭 Microscopes
✨ Fluorophores
🧪 Sample Preparation
🏭 Microscope Brands
🧪 Reagent Suppliers
🔴 Lasers
📷 Detectors
🏛️ Research Organizations (ROR)
Affiliated research institutions:
📊 Figures
Figure 1
Nude mouse visualized 2 years post-injection
a visible light; b. 655nm fluorescence. Despite high intrinsic fluorescence from the intestines, sufficient quantum dot signal exists to distinguish the inguinal and axiliary lymph nodes (indicated by...
Figure 2
Confocal fluorescence images and normalized spectral emission data from frozen lymph node sections from control (24h) and 2yr mice
a projection of 5 spectral slices ranging from 500u2013550nm from a frozen section of a 2yr lymph node pseudo-colored green. This spectral region predominantly shows tissue background autofluorescence...
Figure 3
Fitted quantum emission profile peak positions and spectral widths from lymph node, liver and spleen tissues from control and 2yr mice
a Histogram of fitted peak positions of lymph node, liver and spleen tissues from control (n=3) and 2yr (n=5) mice, b., Plot of fitted peak positions against spectral widths. Note that bars denoted wi...
Figure images are served from the NIH/NLM PubMed Central Open Access Subset or Europe PMC; copyright remains with the publishers and authors.
💬 Discussion
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