Technique • 1486 papers
Abstract Cryo-electron tomography (cryo-ET) has become a key technique for observing the three-dimensional structures of biomolecular complexes and organelles in cellular and tissue environments in...
Biophysical sensing technologies have become central to modern drug discovery because they enable direct, quantitative characterization of ligand-target interactions. In contrast to conventional biochemical and...
We describe a semiautomated approach to segment Env spikes from the membrane envelope of Simian Immunodeficiency Virus visualized by cryoelectron tomography of frozen-hydrated specimens. Multivariate...
Cryo-electron microscopy has become the preferred method for determining structures of macromolecular complexes both in isolation, using single particle analysis, and in their cellular contexts,...
ABSTRACT Most apicomplexan parasites contain a plastid-derived organelle called the apicoplast, which originated through secondary endosymbiosis. As a result of this evolutionary trajectory, the non-photosynthetic...
Abstract Electron cryo-tomography (cryoET) is a powerful imaging tool that allows three-dimensional visualization of subcellular and molecular architecture without chemical fixation. Tomogram quality varies widely,...
Abstract Electron cryo-tomography (cryo-ET) is a powerful imaging tool that allows three-dimensional visualization of subcellular and molecular architecture without chemical fixation. Tomogram quality varies widely,...
Abstract Cryo-electron microscopy (cryo-EM) has transformed structural biology by enabling near-atomic resolution of large macromolecular complexes without the need for crystallization. Here, we describe our...
ABSTRACT Tight junctions (TJs) are composed of anastomosing strands between epithelial cells. Members of the claudin family of proteins reside within TJ strands and either...
Abstract Alzheimer disease (AD), first described by Alzheimer and Perusini in the early twentieth century, is a devastating neurodegenerative disorder without a definitive cure. Unraveling...