Technique • 1376 papers
Abstract Cryo-electron tomography (cryo-ET) has become a key technique for observing the three-dimensional structures of biomolecular complexes and organelles in cellular and tissue environments in...
Abstract The ion permeation pathway is a critical determinant of ion channel function and selectivity; however, the structural basis for ion permeation in the P2X1...
Enteroviruses (EVs) represent a substantial concern to global health. Here, we present the cryo-EM structure of a non-human enterovirus, EV-F4, isolated from the Australian brushtail...
Kir channels are potassium (K+) channels responsible for the mechanism of inward rectification, which plays a fundamental role in maintaining the resting membrane potential. There...
Cryo-electron microscopy has become the preferred method for determining structures of macromolecular complexes both in isolation, using single particle analysis, and in their cellular contexts,...
Many eukaryotic proteins are modified with a polyubiquitin chain and then recruited to either the Cdc48 ATPase (p97 or VCP in mammals) or the 26S...
Abstract Alzheimer disease (AD), first described by Alzheimer and Perusini in the early twentieth century, is a devastating neurodegenerative disorder without a definitive cure. Unraveling...
Cryogenic electron microscopy (cryo-EM) has transformed structural biology by enabling near-atomic resolution imaging of macromolecules and the direct visualization of molecular architectures in native cellular...
Abstract In single particle cryo-EM, analysis of continuous conformational heterogeneity has always been challenging. Both linear and deep learning-based methods treat conformational heterogeneity as perturbations...
Cryogenic electron microscopy (cryo-EM) single-particle analysis (SPA) has become a powerful technique for macromolecular structure determination. However, its effectiveness is often constrained by limited particle...