Technique • 2169 papers
COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast...
ABSTRACT Chiral Au nanocrystals are promising materials for biosensing and therapeutic applications. However, how chirality emerges during their seed‐mediated synthesis remains unclear, leading to limited...
This study was conducted to investigate the ultrastructure of the epithelium and stroma of post-INTACS corneas. INTACS were surgically inserted into the corneas of three...
Abstract Cryo-electron tomography (cryo-ET) has become a key technique for observing the three-dimensional structures of biomolecular complexes and organelles in cellular and tissue environments in...
Cryo-electron microscopy has become the preferred method for determining structures of macromolecular complexes both in isolation, using single particle analysis, and in their cellular contexts,...
ABSTRACT Most apicomplexan parasites contain a plastid-derived organelle called the apicoplast, which originated through secondary endosymbiosis. As a result of this evolutionary trajectory, the non-photosynthetic...
Analysing organelle structures in tissue samples that preserve the in vivo environment, rather than in isolated cells, can provide valuable information on the mechanisms underlying...
Abstract Eukaryotic chromosome segregation requires spindle microtubules to attach to chromosomes through kinetochores. The chromosomal locus that mediates kinetochore assembly is the centromere and is...
Abstract Cryo-electron microscopy (cryo-EM) has transformed structural biology by enabling near-atomic resolution of large macromolecular complexes without the need for crystallization. Here, we describe our...
ABSTRACT Tight junctions (TJs) are composed of anastomosing strands between epithelial cells. Members of the claudin family of proteins reside within TJ strands and either...