Technique • 2561 papers
ABSTRACT Biological research is in constant need of new methodological developments to assess organization and functions at various scales ranging from whole organisms to interactions...
It is challenging to understand viral proteins at a single-molecule level and visualize their dynamics in a cellular environment. This calls for sophisticated microscopy techniques...
Nuclear pore complex (NPC) undergoes dynamic changes in physiology and pathology, yet its roles in neuroblastoma (NB) remain unclear. We demonstrated that rigid extracellular matrix...
Two-dimensional cell culture models have long been a cornerstone of biomedical research; however, they often fail to accurately replicate the in vivo environment. In recent...
Total internal reflection fluorescence microscopy (TIRFM) enables live-cell imaging of clathrin-coated pits (CCPs) but is diffraction-limited (∼250 nm), hindering visualization of their dense nanostructures (∼150 nm). Super-Resolution...
Extracellular vesicles (EVs) facilitate intercellular communication and have emerged as valuable diagnostic and prognostic biomarkers, therapeutic tools, and delivery systems. Due to their heterogeneity, precise...
Super-resolution microscopy bridges the gap between light and electron microscopy and gives new opportunities for the study of proteins that contribute to phloem function. The...
Fluorescence microscopy, especially confocal microscopy, has revolutionized the field of biological imaging. Breaking the optical diffraction barrier of conventional light microscopy, through the advent of...
Neurotransmitter release relies on the regulated fusion of synaptic vesicles (SVs) that are densely packed within the presynaptic bouton of neurons. The mechanisms by which...
ABSTRACT Super‐resolution microscopy (SRM) has exerted a pivotal influence on virology by surpassing the diffraction limits of conventional optical microscopy, enabling unprecedented visualization of viral...