Voltage Imaging

Voltage dynamics of cortical dendrites in vivo.

Voltage dynamics in dendrites, which result both from integrating synaptic inputs and back-propagating action potentials (bAPs) from the soma, contribute to plasticity. Mapping these dynamics...

Bridged-Bicyclic Fluorophores Push Photophysical Boundaries for Live-Cell Imaging.

Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.

Genetically encoded voltage indicators (GEVIs) have long promised optical access to membrane potential, yet their adoption has lagged significantly behind genetically encoded calcium indicators. A...

Large-Scale Neural Recording Technologies for Investigating Circuit Dysfunction and Functional Biomarker Discovery in Animal Models of Alzheimer’s Disease.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder associated with amyloid beta accumulation, tau pathology, and neuronal loss. Increasing evidence suggests that AD is associated...

Retrieving fear memories, as time goes by….

Research in fear conditioning has provided a comprehensive picture of the neuronal circuit underlying the formation of fear memories. In contrast, our understanding of the...

Fast dendritic excitations primarily mediate back-propagation in CA1 pyramidal neurons during behavior.

Abstract Dendrites integrate synaptic inputs to trigger action potentials, and dendrites carry back-propagating action potentials (bAPs) to synapses where these signals contribute to plasticity. Despite...

Fxr1 Deletion from Cortical Parvalbumin Interneurons Modifies Their Excitatory Synaptic Responses.

Fragile X autosomal homolog 1 (FXR1), a member of the fragile X messenger riboprotein 1 family, has been linked to psychiatric disorders including autism and...

High-speed neural imaging with multiplexed miniaturized two-photon microscopy.

Head-mounted miniaturized two-photon microscopes enable cellular-resolution recording of neural activity deep in the mouse brain during unrestrained behavior. Two-photon microscopy, however, is traditionally limited in...

Axons compensate for biophysical constraints of variable size to uniformize their action potentials.

Active conductances tune the kinetics of axonal action potentials (APs) to support specialized functions of neuron types. However, the temporal characteristics of voltage signals strongly...

Voltage imaging with periodic structured illumination.

We utilize periodic structured illumination with pseudo-HiLo (pHiLo) image reconstruction for in vivo voltage imaging. We demonstrate reduced signal from out-of-focus cells, which contaminates voltage...