LSM 710

⭐ Foundational

Imaging small molecule-induced endosomal escape of siRNA.

Nature communications 2020 📊 164

AbstractSmall interfering RNAs (siRNAs) are a new class of promising therapeutic molecules that can be used for sequence-specific downregulation of disease-causing genes. However, endosomal entrapment...

Airyscan MicroscopyConfocal Laser Scanning MicroscopyImmunofluorescence MicroscopyLive Cell ImagingWidefield Fluorescence MicroscopyLSM 710Zeiss Lightsheet Z.1 Light SheetZeiss LSM 710 ConfocalZENAlexa Fluor 647Cy3EGFPGFPHoechst 33342mCherryPropidium IodideYFP3D CultureCell CultureCryosectioningFixationImmunofluorescenceKnockdownLipofectionLive ImagingParaformaldehyde FixationPermeabilizationSpheroidTissue ClearingTransfectionTumor SpheroidEmbryonic Stem CellHeLa (Henrietta Lacks)Human Breast Adenocarcinoma MCF-7Zeiss Lightsheet Z.1 Light SheetZeiss LSM 710 ConfocalCellProfilerFijiHuygensZENExcelGraphPad PrismMATLABRLund UniversitySkåne UniversitySkåne University HospitalUniversity HospitalAddgeneAgilentBD BiosciencesCorningDakoGibcoInvitrogenJackson ImmunoResearchSigma-AldrichThermo Fisher ScientificDonkeyRatHamamatsu ORCA-flash4.0Zeiss AiryscanFilter Set 90Filter Set 92

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⭐ Foundational

ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging.

Scientific reports 2016 📊 196

AbstractUnderstanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing...

Confocal Laser Scanning MicroscopyFluorescence MicroscopyLight Sheet Fluorescence MicroscopyTwo-Photon Excitation MicroscopyZ-Stack ImagingLSM 700LSM 710Zeiss Lightsheet Z.1 Light SheetZeiss LSM 700 ConfocalZeiss LSM 710 ConfocalZeiss LSM 780 ConfocalXenopus laevisXenopus tropicalisZENCy3DAPIGFPAdeno-Associated VirusBlockingClear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hydrogelClear Unobstructed Brain Imaging Cocktails and Computational analysisFixationImmunohistochemistryImmunostainingIn Situ HybridizationParaformaldehyde FixationTissue ClearingZeiss Lightsheet Z.1 Light SheetZeiss LSM 700 ConfocalZeiss LSM 710 ConfocalZeiss LSM 780 Confocalarivis ProAvizoImageJVaa3DZENAligned Genetics, Inc. (South Korea)Korea Brain Research InstituteKorea Institute of Science and TechnologyKorea UniversityUniversity CollegeUniversity College of MedicineLife TechnologiesRocheSigma-AldrichZeiss Plan-Apochromat 10xZeiss Plan-Neofluar 20x

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⭐ Foundational

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9.

Nature communications 2017 📊 221

AbstractImaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for...

Confocal Laser Scanning MicroscopyEpifluorescence MicroscopyFluorescence Recovery After PhotobleachingLattice Light Sheet MicroscopyLight Sheet Fluorescence MicroscopyLive Cell ImagingLSM 710Zeiss LSM 710 ConfocalFijiImageJIN CellMATLABPythonThunderSTORMBFPDAPIGFPmCherryTagBFPYFPCell CultureClustered Regularly Interspaced Short Palindromic RepeatsLentiviralLive ImagingTransductionTransfectionHeLa (Henrietta Lacks)Human Embryonic Kidney 293THuman Osteosarcoma U2OSZeiss LSM 710 ConfocalImageJThunderSTORMIN CellLabVIEWMATLABPythonUniversity of California BerkeleyUniversity of VirginiaAbcamAddgeneLife TechnologiesPromegaTakara BioAndor CCDAndor iXon Ultra 897PMT

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⭐ Foundational

Endolysosomes Are the Principal Intracellular Sites of Acid Hydrolase Activity.

Current biology : CB 2016 📊 230

The endocytic delivery of macromolecules from the mammalian cell surface for degradation by lysosomal acid hydrolases requires traffic through early endosomes to late endosomes followed by...

Confocal Laser Scanning MicroscopyDifferential Interference Contrast MicroscopyFluorescence MicroscopyHigh-Content ScreeningImmunofluorescence MicroscopyTransmission Electron MicroscopyLSM 710Nikon A1 ConfocalTecnaiZeiss LSM 710 ConfocalArrayScanGFPHoechst 33342LysoTrackerLysoTracker GreenTexas RedFixationImmunofluorescencePermeabilizationHeLa (Henrietta Lacks)Human Breast Adenocarcinoma MCF-7Primary Epithelial CellsPrimary FibroblastsNikon A1 ConfocalZeiss LSM 710 ConfocalArrayScanCambridge Institute for Medical Research and Department of Clinical BiochemistryUniversity of CambridgeUniversity of Cambridge School of Clinical MedicineSanta Cruz BiotechnologyThermo Fisher Scientific

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⭐ Foundational

Cortactin promotes exosome secretion by controlling branched actin dynamics.

The Journal of cell biology 2016 📊 287

Exosomes are extracellular vesicles that influence cellular behavior and enhance cancer aggressiveness by carrying bioactive molecules. The mechanisms that regulate exosome secretion are poorly understood....

Confocal Laser Scanning MicroscopyImmunofluorescence MicroscopyTotal Internal Reflection Fluorescence MicroscopyLSM 510LSM 710TecnaiTiZeiss LSM 510 ConfocalZeiss LSM 710 ConfocalDrosophila melanogasterZENAlexa Fluor 546Alexa Fluor 633EGFPFITCGFPmCherrymEGFPmGFPPhalloidinRhodamineFixationGlutaraldehydeImmunofluorescenceImmunostainingKnockdownLentiviralLive ImagingParaformaldehyde FixationPermeabilizationTransfectionHuman Breast Adenocarcinoma MDA-MB-231Zeiss LSM 510 ConfocalZeiss LSM 710 ConfocalDigital MicrographImageJImarisZENExcelGraphPad PrismSPSSKanagawa Cancer CenterThe UniversityUniversity Hospital and ClinicsUniversity Medical SchoolUniversity of TokyoVanderbilt UniversityVanderbilt University Medical CenterVanderbilt University Medical SchoolAbcamAddgeneAgilentGeneTexGibcoInvitrogenMatTekMerckSanta Cruz BiotechnologySigma-AldrichThermo Fisher ScientificMouseRabbitAndor Neo 5.5CCDGatan CCDNikon 100x/1.49 NA

⭐ Foundational

Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contact sites.

The Journal of cell biology 2011 📊 351

Lipid droplets (LDs) are dynamic cellular organelles that control many biological processes. However, molecular components determining LD growth are poorly understood. Genetic analysis has indicated...

Confocal Laser Scanning MicroscopyDifferential Interference Contrast MicroscopyFluorescence Recovery After PhotobleachingImmunofluorescence MicroscopyLive Cell ImagingOptical SectioningZ-Stack ImagingAxiovertLSM 710Zeiss LSM 710 ConfocalMetaMorphBODIPYGFPHoechst 33342Cell CultureFixationGlutaraldehydeImmunofluorescenceKnockdownLentiviralLipofectionLive ImagingMonolayerParaformaldehyde FixationPermeabilizationTransfectionHuman Embryonic Kidney 293TNIH 3T3 Mouse FibroblastZeiss LSM 710 ConfocalImageJUCSF ChimeraMetaMorphExcelTsinghua UniversityInvitrogenSigma-AldrichThermo Fisher Scientific

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⭐ Foundational

mito-QC illuminates mitophagy and mitochondrial architecture in vivo.

The Journal of cell biology 2016 📊 467

Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy...

Airyscan MicroscopyConfocal Laser Scanning MicroscopyFluorescence MicroscopyMultiphoton MicroscopyLSM 700LSM 710LSM 880Zeiss LSM 700 ConfocalZeiss LSM 710 ConfocalZeiss LSM 880 ConfocalZENAlexa Fluor 405Alexa Fluor 633DAPIGFPmCherryMitoTrackerMitoTracker Deep RedRFPBlockingCell CultureCryosectioningFixationImmunohistochemistryImmunolabeling-enabled Three-Dimensional Imaging of Solvent-Cleared OrgansImmunostainingLive ImagingParaformaldehyde FixationPermeabilizationPrimary CultureTransfectionUltramicrotomeWhole MountMouse Embryonic FibroblastZeiss LSM 700 ConfocalZeiss LSM 710 ConfocalZeiss LSM 880 ConfocalVolocityZENZENUniversity of DundeeAbcamBio-RadCell Signaling TechnologyMerckRocheSanta Cruz BiotechnologySigma-AldrichThermo Fisher ScientificVector LaboratoriesChickenMouseRabbitRatSheepZeiss AiryscanZeiss Plan-Apochromat 20xZeiss Plan-Neofluar 40x

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⭐ Foundational

Directional cell movement through tissues is controlled by exosome secretion.

Nature communications 2015 📊 548

Abstract Directional cell movement through tissues is critical for multiple biological processes and requires maintenance of polarity in the face of complex environmental cues. Here...

Confocal Laser Scanning MicroscopyLive Cell ImagingTotal Internal Reflection Fluorescence MicroscopyLSM 510LSM 710TiZeiss LSM 510 ConfocalZeiss LSM 710 ConfocalMetaMorphAlexa Fluor 680DyLightGFPmCherryCell CultureKnockdownLentiviralLive ImagingTransfectionEmbryonic Stem CellZeiss LSM 510 ConfocalZeiss LSM 710 ConfocalFijiMetaMorphVolocityMetaMorphSPSSKanagawa Cancer CenterUniversity Hospital and ClinicsVanderbilt UniversityVanderbilt University Medical CenterAbcamAddgeneBD BiosciencesBio-RadGibcoInvitrogenLife TechnologiesMatTekMilliporeSanta Cruz BiotechnologySigma-AldrichAndor Neo 5.5

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⭐ Foundational

A general method to fine-tune fluorophores for live-cell and in vivo imaging.

Nature methods 2017 📊 546

Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings...

Confocal Laser Scanning MicroscopyDeconvolution MicroscopyFluorescence MicroscopyLight Sheet Fluorescence MicroscopyTwo-Photon Excitation MicroscopyLSM 710LSM 880Zeiss LSM 710 ConfocalZeiss LSM 880 ConfocalDrosophila melanogasterFijiHALOImageJLAS XMATLABGCaMP6sHoechst 33342mCherryRFPRhodamineAdeno-Associated VirusCell CultureFixationLipofectionTransfectionAfrican Green Monkey Kidney COS-7Human Osteosarcoma U2OSZeiss LSM 710 ConfocalZeiss LSM 880 ConfocalImageJLAS XMATLABHoward Hughes Medical InstituteJanelia Research CampusAgilentLife TechnologiesNew England BiolabsPromegaThermo Fisher ScientificCoherent ChameleonCoherent Chameleon UltraSpectra-Physics InSight DeepSeeTi:Sapphire25x/1.05 NAZeiss C-APO 40×Zeiss Plan APO 20×

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⭐ Foundational

Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown.

Nature biotechnology 2015 📊 604

A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the...

Calcium ImagingConfocal Laser Scanning MicroscopyEpifluorescence MicroscopyLive Cell ImagingSpinning Disk Confocal MicroscopyA1Axio ObserverLSM 710Nikon A1 ConfocalZeiss LSM 710 ConfocalSlideBookAlexa Fluor 647Cy3DsRedEGFPFluo-4GFPmCherrymRFPYFPCell CultureFixationKnockdownLipofectionLive ImagingTransductionTransfectionEmbryonic Stem CellHeLa (Henrietta Lacks)Nikon A1 ConfocalZeiss LSM 710 ConfocalCellProfilerSlideBookMATLABBoston Children's HospitalHarvard Medical SchoolLund Universitys HospitalSemmelweis UniversityAddgeneBio-RadGibcoLife TechnologiesLonzaR&D SystemsSigma-AldrichHamamatsu EMCCD