N-SIM

N-SIM

Microscope • 58 papers

⭐ Foundational

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.

Frontiers in molecular neuroscience 2013 📊 731

Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, "RCaMPs," engineered from circular permutation of the thermostable...

Calcium ImagingConfocal Laser Scanning MicroscopyDifferential Interference Contrast MicroscopyFluorescence Correlation SpectroscopyFluorescence MicroscopyOptogeneticsAxio ObserverN-SIMCaenorhabditis elegansDrosophila melanogasterEscherichia coliFijiGraphPad PrismImageJMATLABCy3EdUEGFPGCaMPGFPmCherrymRubyOregon Green BAPTAR-GECORCaMPRFPAdeno-Associated VirusCo-cultureLentiviralTransductionTransfectionHuman Embryonic Kidney 293Primary AstrocytesAxio ObserverN-SIMImageJGraphPad PrismLabVIEWMATLABHoward Hughes Medical InstituteJanelia FarmCorningInvitrogenLife TechnologiesLonzaMilliporeQiagenRocheTakara BioCoherent ChameleonTi:SapphireAndor EMCCDAPDCCDPhotometrics CCDPhotometrics CoolSnapPicoQuant APDPrinceton Instruments CCDRoper APDRoper CCDSemrock APD40x/0.8 NA60x/1.2 NAZeiss 20x/0.85 NAZeiss Plan-Neofluar 25x647/57 bandpassSemrock FF01-468
💾 Data
⭐ Foundational

Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis.

Nature 2018 📊 784

Both mitochondria and lysosomes are essential for maintaining cellular homeostasis, and dysfunction of both organelles has been observed in multiple diseases. Mitochondria are highly dynamic...

Confocal Laser Scanning MicroscopyCorrelative Light and Electron MicroscopyFörster Resonance Energy TransferImmunofluorescence MicroscopyLive Cell ImagingSpinning Disk Confocal MicroscopyStructured Illumination MicroscopyTransmission Electron MicroscopyZ-Stack ImagingA1FEI TecnaiN-SIMNikon A1 ConfocalNikon N-SIMTecnaiNIS-ElementsBFPEGFPGFPLysoTrackerLysoTracker RedmApplemCherrymClovermEmeraldmGFPmRubymTurquoisemVenusRFPVenusYFPCell CultureFixationGlutaraldehydeImmunofluorescenceLipofectionLive ImagingPermeabilizationTransfectionUltramicrotomeHeLa (Henrietta Lacks)Human Colorectal Carcinoma HCT116Human Embryonic Kidney 293Nikon A1 ConfocalImageJNIS-ElementsNorthwestern UniversityUniversity Feinberg School of MedicineAddgeneBD BiosciencesCell Signaling TechnologyDakoGibcoInvitrogenLife TechnologiesMatTekSigma-AldrichThermo Fisher ScientificFEI CCDNikon GaAsPNikon 100x/1.45 NA oil
⭐ Foundational

In vivo three-photon microscopy of subcortical structures within an intact mouse brain.

Nature photonics 2013 📊 1,393

Two-photon fluorescence microscopy (2PM)1 enables scientists in various fields including neuroscience2,3, embryology4, and oncology5 to visualize in vivo and ex vivo tissue morphology and physiology...

Fluorescence MicroscopyMultiphoton MicroscopyOptical SectioningThree-Photon MicroscopyN-SIMScanImageRFPTexas RedN-SIMMATLABYOLOvScanImageMATLABCornell UniversityInvitrogenHamamatsu GaAsPHamamatsu PMTPMTSutter PMT
💾 Data

MicroHub Assistant