Nikon N-SIM Microscopy Papers

Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor Golgi.

Biology open 2025 📊 2

ABSTRACT Rod photoreceptor stability is critical for retinal health and lifelong vision. Rhodopsin (Rho) trafficking is essential for rod homeostasis, as its mislocalization precedes rod...

🏷️ 8 RRIDs

Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor Golgi.

bioRxiv : the preprint server for biology 2025

Abstract Rod photoreceptor stability is critical for retinal health and lifelong vision. The proper intracellular trafficking of the photopigment receptor rhodopsin (Rho) is essential for...

Confocal Laser Scanning Microscopy Immunofluorescence Microscopy Spinning Disk Confocal Microscopy Stochastic Optical Reconstruction Microscopy Structured Illumination Microscopy Super-Resolution Microscopy Total Internal Reflection Fluorescence Microscopy Nikon N-SIM Nikon N-STORM NIS-Elements Alexa Fluor 647 CF568 DAPI EGFP GFP RFP TagRFP TagRFP-T WGA Adeno-Associated Virus Antigen Retrieval Blocking Cell Culture Fixation Glutaraldehyde Immunofluorescence Immunostaining Knockdown Knockout Paraformaldehyde Fixation Permeabilization Transduction Transfection Ultramicrotome Vibratome Embryonic Stem Cell Human Embryonic Kidney 293T Nikon N-SIM Nikon N-STORM Fiji ImageJ NIS-Elements GraphPad Prism Mathematica Wolfram University School of Medicine Virginia University Addgene BD Biosciences Bio-Rad BioLegend Biotium GeneTex Gibco Invitrogen Lonza Millipore New England Biolabs Sigma-Aldrich Thermo Fisher Scientific VWR Goat Mouse Rabbit Andor Ixon Ultra Hamamatsu Orca Flash 4.0 Hamamatsu sCMOS 60x/1.42 NA oil Apochromat TIRF 100X

🏷️ 8 RRIDs

Structural remodeling of target-SNARE protein complexes by NSF enables synaptic transmission.

bioRxiv : the preprint server for biology 2025 📊 1

Abstract Synaptic vesicles containing neurotransmitters fuse with the plasma membrane upon the arrival of an action potential at the active zone. Multiple proteins organize trans-SNARE...

💾 Data

P23H rhodopsin aggregation in the ER causes synaptic protein imbalance in rod photoreceptors.

bioRxiv : the preprint server for biology 2024

Abstract Rod photoreceptor neurons in the retina detect scotopic light through the visual pigment rhodopsin (Rho) in their outer segments (OS). Efficient Rho trafficking to...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Immunofluorescence Microscopy Spinning Disk Confocal Microscopy Structured Illumination Microscopy Transmission Electron Microscopy Nikon AX Nikon N-SIM Mus musculus NIS-Elements Alexa Fluor 647 DAPI EGFP GFP mCherry RFP TagRFP TagRFP-T WGA Adeno-Associated Virus Antigen Retrieval Blocking Clustered Regularly Interspaced Short Palindromic Repeats Cryosectioning Fixation Glutaraldehyde Hematoxylin and Eosin Immunofluorescence Immunostaining Paraformaldehyde Fixation RNAscope Transduction Ultramicrotome Vibratome Nikon AX Nikon N-SIM Fiji ImageJ NIS-Elements GraphPad Prism Virginia University Abcam Bio-Rad Biotium Cell Signaling Technology Invitrogen Lonza Millipore New England Biolabs Sigma-Aldrich Thermo Fisher Scientific VWR Goat Mouse Rabbit Hamamatsu Orca Flash 4.0 Hamamatsu sCMOS 100x/1.45 NA oil 40x/1.30 NA 60x/1.42 NA oil Apochromat TIRF 100X

💾 Data 🏷️ 19 RRIDs

Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells.

Methods in molecular biology (Clifton, N.J.) 2022 📊 2

Budding yeast, Saccharomyces cerevisiae, is an appealing model organism to study the organization and function of the actin cytoskeleton. With the advent of techniques to...

Colocalization and Interaction Study of Neuronal JNK3, JIP1, and β-Arrestin2 Together with PSD95.

International journal of molecular sciences 2022 📊 3

c-Jun N-terminal kinases (JNKs) are stress-activated serine/threonine protein kinases belonging to the mitogen-activated protein kinase (MAPK) family. Among them, JNK3 is selectively expressed in the...

Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications.

Cells 2023 📊 3

The modern fluorescence microscope is the convergence point of technologies with different performances in terms of statistical sampling, number of simultaneously analyzed signals, and spatial...

Confocal Laser Scanning Microscopy Direct Stochastic Optical Reconstruction Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Single-Molecule Imaging Stochastic Optical Reconstruction Microscopy Structured Illumination Microscopy Super-Resolution Microscopy Total Internal Reflection Fluorescence Microscopy Widefield Fluorescence Microscopy Z-Stack Imaging Nikon N-SIM Nikon N-STORM NIS-Elements Alexa Fluor 488 Alexa Fluor 647 Click-iT Cy3 DAPI EdU Hoechst 33342 Pacific Blue Pacific Orange Phalloidin Blocking Cell Culture Fixation Immunofluorescence Immunostaining Permeabilization Human Mammary Epithelial MCF-10A Nikon N-SIM Nikon N-STORM ImageJ NIS-Elements European Institute of Oncology IRCCS University of Milan Abcam BD Biosciences BioLegend MatTek Merck R&D Systems Sigma-Aldrich Thermo Fisher Scientific Donkey Goat Mouse Rabbit Rat GaAsP Hamamatsu Orca Flash 4.0 100x/1.49 NA Apochromat TIRF 100X Chroma 561/647 bandpass

💻 Code 💾 Data

Structural remodeling of target-SNARE protein complexes by NSF enables synaptic transmission.

Nature communications 2025 📊 4

Abstract Synaptic vesicles containing neurotransmitters fuse with the plasma membrane upon the arrival of an action potential at the active zone. Multiple proteins organize trans-SNARE...

💾 Data

P23H rhodopsin accumulation causes transient disruptions to synaptic protein levels in rod photoreceptors in a model of retinitis pigmentosa.

Disease models & mechanisms 2025 📊 4

ABSTRACT Rod photoreceptor neurons in the retina detect scotopic light through the visual pigment rhodopsin (Rho) in their outer segment (OS). Efficient Rho trafficking to...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Immunofluorescence Microscopy Spinning Disk Confocal Microscopy Structured Illumination Microscopy Transmission Electron Microscopy Nikon AX Nikon N-SIM Mus musculus NIS-Elements Alexa Fluor 647 DAPI EGFP GFP mCherry RFP TagRFP TagRFP-T WGA Adeno-Associated Virus Antigen Retrieval Blocking Clustered Regularly Interspaced Short Palindromic Repeats Cryosectioning Fixation Glutaraldehyde Hematoxylin and Eosin Immunofluorescence Immunostaining Paraformaldehyde Fixation RNAscope Transduction Ultramicrotome Vibratome Nikon AX Nikon N-SIM Fiji ImageJ NIS-Elements GraphPad Prism Virginia University Abcam Bio-Rad Biotium Cell Signaling Technology Invitrogen Lonza Millipore New England Biolabs Sigma-Aldrich Thermo Fisher Scientific Vector Laboratories VWR Goat Mouse Rabbit Hamamatsu Orca Flash 4.0 Hamamatsu sCMOS 100x/1.45 NA oil 100x/1.49 NA oil 40x/1.30 NA 60x/1.42 NA oil

💾 Data 🏷️ 20 RRIDs

Super-resolution study of PIAS SUMO E3-ligases in hippocampal and cortical neurons.

European journal of histochemistry : EJH 2021 📊 5

The SUMOylation machinery is a regulator of neuronal activity and synaptic plasticity. It is composed of SUMO isoforms and specialized enzymes named E1, E2 and...

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