Technique • 100 papers
Abstract Imaging subcellular structures deep within thick, turbid biological tissues remains fundamentally limited by light scattering, which distorts optical wavefronts and degrades contrast, resolution, and...
Animal models are essential for defining mechanisms of HIV-associated brain injury in the antiretroviral therapy era, where neurocognitive impairment and brain aging have replaced opportunistic...
Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature...
Three-photon fluorescence microscopy (3PM) has driven rapid progress in deep-tissue imaging beyond the depth limit of two-photon microscopy, with impacts in neuroscience, immunology, and cancer...
Three-photon microscopy (3PM) has emerged as a versatile intravital imaging technology, enabling analysis of cell dynamics and interactions with the tissue microenvironment beyond the capabilities...
Three-photon microscopy (3PM) allows deep-tissue imaging beyond the capabilities of two-photon microscopy (2PM) owing to infrared excitation. Here, we present a protocol for time-lapse 3D...
This review addresses the challenges of obtaining high-quality quantitative data in the optical imaging of membrane voltage and calcium dynamics. The paper provides a comprehensive...
The lifelong addition of stem-cell-derived neurons into distinct areas of the mammalian brain, such as the olfactory bulb and hippocampal dentate gyrus, provides structural and...
AbstractIdentifying initial events of mucosal entry of human immunodeficiency virus type-1 (HIV-1) in laboratory-based, physiologically relevant and high-throughput contexts may aid in designing effective strategies...