Technique • 29 papers
Here, we present a protocol for preserving fluorescent markers using tissue clearing and immunolabeling in mouse tissue such as sciatic nerve, gut, and spinal cord....
Optical clearing combined with light-sheet microscopy enables high-resolution imaging of extended tissue at scale. However, standard mesoSPIM systems are optimised for intact organs and are...
Although several open-source, easy-to-assemble light-sheet microscope platforms already exist—such as mesoSPIM, OpenSPIM, and OpenSpin—they are optimized for imaging large specimens and lack the resolution required...
The number of grey values that can be displayed on monitors and be processed by the human eye is smaller than the dynamic range of...
Summary CRISPR-based genome engineering holds enormous promise for basic science and therapeutic applications. Integrating and editing DNA sequences is still challenging in many cellular contexts,...
Abstract Light-field imaging is an emerging paradigm in biomedical optics, offering the unique ability to capture volumetric information in a single snapshot by encoding both...
Background Volumetric atlases are an invaluable tool in neuroscience and otolaryngology, greatly aiding experiment planning and surgical interventions, as well as the interpretation of experimental...
OBJECTIVE: Aging results in significant structural and functional changes in the anterior segment of the eye, influencing intraocular pressure (IOP) and overall ocular health. Although...
Feeding is an innate behavior critical for survival but is also influenced by many non-nutritional factors such as emotion, social context and environmental conditions. Recently,...