Microscope • 53 papers
Fluorescent biosensors toggle between two states, and for the vast majority of biosensors, one state is bright and the other state is dim. As a...
Myeloid phagocytes are essential for antifungal host defense during systemic candidiasis. Here, we present a protocol for assessing phagocyte-fungal interactions in vivo in the kidney, the...
Expansion Microscopy (ExM) is a powerful method that enables super-resolution fluorescence microscopy, beyond the diffraction limit of light, with conventional fluorescence microscopes. Briefly, macromolecules of...
Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using...
Polyploidy (whole-genome duplication) is a common yet under-surveyed property of tissues across multicellular organisms. Polyploidy plays a critical role during tissue development, following acute stress,...
Many viruses use programmed frameshifting and stop-codon misreading to synthesize functional proteins at high levels. The underlying mechanisms involve complex RNA sequence/structure motifs and likely...
Expansion microscopy (ExM) enables nanoscale imaging of biological structures using standard fluorescence microscopes. Accurate labeling of cytoskeletal filaments, such as microtubules, remains challenging due to...
Chromatin exhibits non-random distribution within the nucleus being arranged into discrete domains that are spatially organized throughout the nuclear space. Both the spatial distribution and...
ABSTRACT Many gram-negative bacteria utilize a type III secretion system (T3SS) injectisome to translocate effector proteins directly into host cells. In Bordetella bronchiseptica , a...
ABSTRACT The virus factories (VFs) of infectious bursal disease virus (IBDV) are biomolecular condensates formed through liquid-liquid phase separation (LLPS). A major component of the...