Technique • 135 papers
The mechanism of molecular passage through nuclear pore complexes (NPCs) remains debated between passive diffusion and constrained transport. Integrating single-particle tracking, cargo-centric ensemble assays, and...
Abstract Nuclear envelope (NE) reformation after mitosis is essential for daughter cell viability and requires tightly coordinated nuclear pore complex (NPC) assembly and nuclear membrane...
ABSTRACT Long-distance transport in neurons relies on motor proteins that can move towards either the plus- or minus-end of microtubules. In axons, microtubules uniformly have...
Single-molecule localization microscopy (SMLM) has transformed biological imaging by enabling nanoscale visualization of intricate subcellular structures. However, conventional three-dimensional SMLM techniques typically exhibit lower axial...
MINimal photon FLUXes (MINFLUX) offers nanometer localization precision, with lower fluorophore requirements than for other super-resolution microscopy (SRM) techniques. Nonetheless, low localization probabilities hamper its...
Abstract The maturation speed of fluorescent proteins is a crucial parameter that influences cellular brightness, effective labeling efficiency, and temporal resolution in fluorescence microscopy. Green‐to‐red...
To understand the mechanics and kinetic properties of cytoskeletal molecular motors such as myosin, typically the motor of interest needs to be expressed and purified...
Abstract Recent advances in fluorescence nanoscopy have pushed resolution to the 1–10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments....
Abstract The cardiac ryanodine receptor (RyR2) constitutes the molecular basis of the process of calcium-induced calcium release where activation of RyR2s can be locally regenerative....