CSU-X1 Microscopy Papers – Page 4

⭐ Foundational

Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens.

Cell 2012 📊 294

Optical imaging of the dynamics of living specimens involves tradeoffs between spatial resolution, temporal resolution, and phototoxicity, made more difficult in three dimensions. Here, however,...

💾 Data

⭐ Foundational

NETosis proceeds by cytoskeleton and endomembrane disassembly and PAD4-mediated chromatin decondensation and nuclear envelope rupture.

Proceedings of the National Academy of Sciences of the United States of America 2020 📊 364

Neutrophil extracellular traps (NETs) are web-like DNA structures decorated with histones and cytotoxic proteins that are released by activated neutrophils to trap and neutralize pathogens...

Confocal Laser Scanning Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Live Cell Imaging Spinning Disk Confocal Microscopy Widefield Fluorescence Microscopy A1 Axiovert CSU-W1 CSU-X1 LSM 510 Nikon A1 Confocal Ti Ti2 Yokogawa CSU-W1 Spinning Disk Yokogawa CSU-X1 Yokogawa CSU-X1 Spinning Disk Zeiss Axiovert Zeiss LSM 510 Zeiss LSM 510 Confocal Excel Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 Calcein DAPI EGFP ER-Tracker ER-Tracker Red GFP mApple mCherry mEmerald SiR-Actin SiR-Tubulin SYTOX SYTOX Green Fixation Immunofluorescence Live Imaging Permeabilization Human Promyelocytic Leukemia HL-60 Primary Neutrophils Nikon A1 Confocal Yokogawa CSU-W1 Spinning Disk Yokogawa CSU-X1 Spinning Disk Zeiss LSM 510 Confocal Excel Boston Children's Hospital Harvard Medical School National Institutes of Health NIH Northwestern University s Hospital University Feinberg School of Medicine University of Texas Southwestern Medical Center

💾 Data

⭐ Foundational

Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture.

Cell death and differentiation 2019 📊 340

Pyroptosis is rapidly emerging as a mechanism of anti-microbial host defense, and of extracellular release of the inflammasome-dependent cytokines interleukin (IL)-1β and IL-18, which contributes...

⭐ Foundational

Nanoscale imaging of RNA with expansion microscopy.

Nature methods 2016 📊 373

The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in...

Confocal Laser Scanning Microscopy Epifluorescence Microscopy Expansion Microscopy Light Sheet Fluorescence Microscopy Optical Sectioning Spinning Disk Confocal Microscopy CSU-X1 Nikon Ti SP8 Stellaris Ti Yokogawa CSU-X1 Spinning Disk Zeiss Lightsheet Z.1 Light Sheet NIS-Elements ZEN ATTO 647N Cy5 EdU FITC GFP QuasAr YFP Cell Culture Expansion Microscopy Fixation Fluorescence In Situ Hybridization In Situ Hybridization Single-Molecule Fluorescence In Situ Hybridization Vibratome HeLa (Henrietta Lacks)Human Embryonic Kidney 293 Yokogawa CSU-X1 Spinning Disk Zeiss Lightsheet Z.1 Light Sheet arivis Pro arivis Vision4D Fiji Imaris NIS-Elements ZEN MATLAB Harvard Medical School Massachusetts Institute of Technology University of Pennsylvania Wyss Institute for Biologically Inspired Engineering Invitrogen Life Technologies New England Biolabs Andor sCMOS PCO sCMOS PCO.Edge 5.sCMOS 40x/1.15 NA water 4x/0.2 NA air 60x/1.4 NA oil 525/40 bandpass 607/36 bandpass 685/40 bandpass

💾 Data

⭐ Foundational

Locally renewing resident synovial macrophages provide a protective barrier for the joint.

Nature 2019 📊 528

Macrophages are considered to contribute to chronic inflammatory diseases such as rheumatoid arthritis1. However, both the exact origin and the role of macrophages in inflammatory...

Brightfield Microscopy Confocal Laser Scanning Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Light Sheet Fluorescence Microscopy Spinning Disk Confocal Microscopy Transmission Electron Microscopy Axio Observer CSU-X1 LaVision BioTec UltraMicroscope Light Sheet Observer 7 Ti UltraMicroscope ZEN ZEN Blue Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 700 APC BV421 Cy5 Cy5.5 DAPI DsRed EdU EGFP FITC GFP mCherry PerCP Phalloidin SYTOX SYTOX Blue tdTomato Blocking Cryosectioning Fixation Glutaraldehyde Immunofluorescence Microtome Optimal Cutting Temperature Embedding Paraformaldehyde Fixation Permeabilization Tissue Clearing Primary Endothelial Cells Primary Macrophages Primary Monocytes Primary Neutrophils LaVision BioTec UltraMicroscope Light Sheet Huygens Imaris ZEN ZEN Blue GraphPad Prism Alexander University Institut de Génétique Humaine Leibniz Research Institute of Molecular Pharmacology Research Institute of Radiology Spanish National Centre for Cardiovascular Research Universitätsklinikum Erlangen BioLegend Dako Invitrogen Qiagen Sigma-Aldrich Donkey Rabbit Rat Andor sCMOS Leica HyD Photometrics Evolve 512 Yokogawa EMCCD Leica HCX PL APO 100x oil Zeiss LD C-Apochromat 63x water 445/50 bandpass 525/50 bandpass 605/70 bandpass

💾 Data

⭐ Foundational

Fast and sensitive GCaMP calcium indicators for imaging neural populations.

Nature 2023 📊 656

Abstract Calcium imaging with protein-based indicators 1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity...

Calcium Imaging Confocal Laser Scanning Microscopy Fluorescence Correlation Spectroscopy Fluorescence Recovery After Photobleaching Immunofluorescence Microscopy Spinning Disk Confocal Microscopy Two-Photon Excitation Microscopy Axio Imager Axio Observer CSU-X1 Nikon Ti Ti Yokogawa CSU-X1 Spinning Disk Zeiss Axio Observer Drosophila melanogaster Escherichia coli MetaMorph ScanImage Alexa Fluor 488 Alexa Fluor 594 Cy3 DAPI EdU EGFP GCaMP GCaMP6 GCaMP6s GFP jGCaMP7 jGCaMP8 mCherry mEmerald RFP tdTomato Adeno-Associated Virus Cell Culture Electroporation Fixation Immunofluorescence Immunostaining Paraformaldehyde Fixation Transduction Transfection Embryonic Stem Cell Primary Neurons Yokogawa CSU-X1 Spinning Disk ilastik PyMOL MetaMorph ScanImage Excel MATLAB Python Allen Institute Allen Institute for Neural Dynamics HHMI Howard Hughes Medical Institute Janelia Research Campus Ministry of Defence Princeton University University of California University of Maryland School of Medicine Addgene Agilent Bio-Rad Invitrogen Lonza MatTek Millipore New England Biolabs Sigma-Aldrich Takara Bio Thermo Fisher Scientific Tocris Chicken Goat Mouse Rabbit Rat Coherent Chameleon Ultra Spectra-Physics Mai Tai Ti:Sapphire Andor EMCCD Andor iXon 897 Andor Ixon Ultra EMCCD GaAsP Nikon EMCCD

💻 Code 💾 Data

⭐ Foundational

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.

Nature biotechnology 2016 📊 584

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes...

Confocal Laser Scanning Microscopy Epifluorescence Microscopy Expansion Microscopy Fluorescence Microscopy Live Cell Imaging Optical Sectioning Photoactivated Localization Microscopy Spinning Disk Confocal Microscopy Structured Illumination Microscopy CSU-X1 LSM 710 Nikon Ti Ti Yokogawa CSU-X1 Spinning Disk Zeiss LSM 710 Zeiss LSM 710 Confocal Macaca mulatta NIS-Elements CF633 Dendra2 EGFP GFP iRFP mClover mEmerald mKate2 mRuby Adeno-Associated Virus Blocking Cell Culture Expansion Microscopy Fixation Immunostaining Live Imaging Microtome Paraformaldehyde Fixation Permeabilization Transfection Vibratome HeLa (Henrietta Lacks)Primary Neurons Yokogawa CSU-X1 Spinning Disk Zeiss LSM 710 Confocal CellProfiler NIS-Elements Igor Pro MATLAB Howard Hughes Medical Institute Janelia Research Campus Massachusetts Institute of Technology Massachusetts Institute of Technology Media Lab MIT Osaka University Osaka University Medical School University Medical School University of Michigan University of Michigan Medical School Abcam Addgene Agilent Biotium Invitrogen Life Technologies New England Biolabs Promega Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific Chicken Donkey Goat Rabbit Rat Sheep Andor EMCCD Andor Ixon Ultra 10x/0.45 NA 40x/1.15 NA water 4x/0.13 NA 4x/0.13 NA air 60x/1.40 NA oil Olympus 100x/1.40 NA Zeiss 10x/0.3 NA air Zeiss 40x/1.1 NA water Chroma FF01-609

CSU-X1

Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens.

NETosis proceeds by cytoskeleton and endomembrane disassembly and PAD4-mediated chromatin decondensation and nuclear envelope rupture.

Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture.

Nanoscale imaging of RNA with expansion microscopy.

Locally renewing resident synovial macrophages provide a protective barrier for the joint.

Fast and sensitive GCaMP calcium indicators for imaging neural populations.

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.

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