CSU-W1 Microscopy Papers

🔥 High Impact

Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis.

Development (Cambridge, England) 2019 📊 82

ABSTRACT Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous...

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Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell-target interactions.

Nature communications 2020 📊 137

Abstract Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements...

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The Nutrient-Responsive Hormone CCHamide-2 Controls Growth by Regulating Insulin-like Peptides in the Brain of Drosophila melanogaster.

PLoS genetics 2015 📊 181

The coordination of growth with nutritional status is essential for proper development and physiology. Nutritional information is mostly perceived by peripheral organs before being relayed...

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The Evolution of Erythrocytes Becoming Red in Respect to Fluorescence.

Frontiers in physiology 2019 📊 6

Very young red blood cells, namely reticulocytes, can be quite easily recognized and labeled by cluster of differentiation antibodies (CD71, transferrin receptor) or by staining...

🔥 High Impact

Bright and stable monomeric green fluorescent protein derived from StayGold.

Nature methods 2024 📊 81

The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Expansion Microscopy Fluorescence Microscopy Live Cell Imaging Spinning Disk Confocal Microscopy Structured Illumination Microscopy Widefield Fluorescence Microscopy Z-Stack Imaging Axio Imager CSU-W1 LSM 980 N-SIM Nikon Ti2 Ti Ti2 Yokogawa CSU-W1 Spinning Disk Zeiss Axio Imager Zeiss LSM 980 Zeiss LSM 980 Confocal Caenorhabditis elegans Escherichia coli NIS-Elements ZEN ZEN Blue Coumarin EdU EGFP FITC FusionRed GFP mCherry mClover mEGFP mKate2 mNeonGreen mScarlet mTagBFP RFP TRITC Venus Cell Culture Expansion Microscopy Fixation Lipofection Live Imaging Paraformaldehyde Fixation Transfection African Green Monkey Kidney COS-7 HeLa (Henrietta Lacks)Yokogawa CSU-W1 Spinning Disk Zeiss LSM 980 Confocal Fiji ImageJ NIS-Elements ZEN ZEN Blue Excel Python CAS Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences McGovern Institute for Brain Research Middle Tennessee State University Moscow Institute of Physics and Technology National Research Center Ovchinnikov Institute of Bioorganic Chemistry Peking University Russian Academy of Sciences State University Westlake Institute for Advanced Study Westlake Laboratory of Life Sciences and Biomedicine Westlake University Addgene BD Biosciences Bio-Rad Gibco Invitrogen MatTek New England Biolabs Qiagen Sigma-Aldrich Takara Bio Thermo Fisher Scientific Hamamatsu Orca Flash 4.0 Olympus sCMOS sCMOS Semrock sCMOS 100x/1.5 NA oil Zeiss 63x/1.4 NA oil 525/50 bandpass 535/40 bandpass 535/50 bandpass Leica 405/40 bandpass Leica 450/40 bandpass Leica 480/40 bandpass Nikon 470/40 bandpass Nikon 525/50 bandpass Semrock 470/28 bandpass

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⭐ Foundational

FiloQuant reveals increased filopodia density during breast cancer progression.

The Journal of cell biology 2017 📊 129

Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological...

Confocal Laser Scanning Microscopy Immunofluorescence Microscopy Intravital Microscopy Live Cell Imaging Spinning Disk Confocal Microscopy Structured Illumination Microscopy Total Internal Reflection Fluorescence Microscopy Z-Stack Imaging Axio Observer CSU-W1 LSM 780 Observer 7 Yokogawa CSU-W1 Yokogawa CSU-W1 Spinning Disk Zeiss Axio Observer Zeiss LSM 780 Confocal Danio rerio SlideBook ZEN Alexa Fluor 488 Alexa Fluor 568 DAPI EGFP GFP mCherry mEmerald mRFP Phalloidin RFP Cell Culture Fixation Immunofluorescence Lentiviral Lipofection Live Imaging Monolayer Paraformaldehyde Fixation Permeabilization Spheroid Transduction Transfection Tumor Spheroid Human Breast Adenocarcinoma MDA-MB-231 Human Mammary Epithelial MCF-10A Primary Epithelial Cells Primary Natural Killer Cells Primary Neurons Yokogawa CSU-W1 Spinning Disk Zeiss LSM 780 Confocal Fiji ImageJ Imaris TrackMate SlideBook ZEN Akademi University Baylor College of Medicine s Hospital Texas Children's Hospital Turku Centre for Biotechnology University of Turku University of Turku and Åbo Akademi University Addgene BD Biosciences BioLegend Cytiva Gibco ibidi Invitrogen Life Technologies MatTek Merck Roche Sigma-Aldrich Thermo Fisher Scientific EMCCD Hamamatsu C9100-13 Hamamatsu EMCCD Hamamatsu Orca Flash 4 Hamamatsu sCMOS PCO sCMOS Photometrics Evolve 512

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⭐ Foundational

Cell fate coordinates mechano-osmotic forces in intestinal crypt formation.

Nature cell biology 2021 📊 167

Intestinal organoids derived from single cells undergo complex crypt-villus patterning and morphogenesis. However, the nature and coordination of the underlying forces remains poorly characterized. Here,...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Light Sheet Fluorescence Microscopy Live Cell Imaging Spinning Disk Confocal Microscopy Z-Stack Imaging Axio Observer CSU-W1 LSM 710 Ti Ti2 Yokogawa CSU-W1 Spinning Disk Yokogawa CV7000 Zeiss Axio Observer Zeiss LSM 710 Confocal Drosophila melanogaster ZEN ZEN Black DAPI GFP Blocking Cell Culture Fixation Immunostaining Intestinal Organoid Live Imaging Paraformaldehyde Fixation Transduction Madin-Darby Canine Kidney Yokogawa CSU-W1 Spinning Disk Zeiss LSM 710 Confocal Fiji Huygens ImageJ MorphoGraphX scikit-image U-Net ZEN ZEN Black Python R European Molecular Biology Laboratory Institute of Science and Technology Austria National University of Singapore University of Basel Abcam Bio-Rad Corning ibidi Invitrogen R&D Systems Sigma-Aldrich Thermo Fisher Scientific Tocris Cobolt Jive Coherent Chameleon diode DPSS Ti:Sapphire Toptica iBeam smart Toptica iBeam Smart 405 Andor sCMOS Zeiss Plan-Neofluar 40x

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⭐ Foundational

Structure of the human epithelial sodium channel by cryo-electron microscopy.

eLife 2018 📊 147

The epithelial sodium channel (ENaC), a member of the ENaC/DEG superfamily, regulates Na+ and water homeostasis. ENaCs assemble as heterotrimeric channels that harbor protease-sensitive domains...

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⭐ Foundational

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT.

Nature communications 2017 📊 152

AbstractSingle-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine...

Confocal Laser Scanning Microscopy DNA Points Accumulation for Imaging in Nanoscale Topography Optical Sectioning Single-Molecule Localization Microscopy Spinning Disk Confocal Microscopy Super-Resolution Microscopy Total Internal Reflection Fluorescence Microscopy CSU-W1 Ti Yokogawa CSU-W1 Spinning Disk Dragonfly DAPI Fixation Fluorescence In Situ Hybridization Glutaraldehyde Immunostaining Paraformaldehyde Fixation Permeabilization HeLa (Henrietta Lacks)Human Fetal Lung Fibroblast IMR-90 Human Fetal Lung Fibroblast WI-38 Mouse Embryonic Fibroblast Yokogawa CSU-W1 Spinning Disk Dragonfly Harvard University Max Planck Max Planck Institute Max Planck Institute of Biochemistry Maximilian University Modelica Association Wyss Institute for Biologically Inspired Engineering Abcam Bio-Rad Gibco ibidi Invitrogen Jackson ImmunoResearch New England Biolabs Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific VWR Cobolt Samba coherent sapphire or diode DPSS Andor iXon 888 Andor iXon 897 Andor Zyla 4.2 EMCCD sCMOS Plan Apo 60x Chroma ET585/65m Semrock Di01-T405

⭐ Foundational

Improved split fluorescent proteins for endogenous protein labeling.

Nature communications 2017 📊 292

Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact. To expand this...

Confocal Laser Scanning Microscopy Fluorescence Microscopy Live Cell Imaging Stochastic Optical Reconstruction Microscopy Super-Resolution Microscopy Widefield Fluorescence Microscopy CSU-W1 Nikon Ti Ti Yokogawa CSU-W1 Yokogawa CSU-W1 Spinning Disk Escherichia coli MicroManager GFP LysoTracker LysoTracker Blue mEmerald sfGFP Split-GFP TagBFP Cell Culture Electroporation Fixation Lentiviral Live Imaging Transfection HeLa (Henrietta Lacks)Human Embryonic Kidney 293 Human Embryonic Kidney 293T Primary T Cells Yokogawa CSU-W1 Spinning Disk Fiji ImageJ MicroManager GraphPad Prism Howard Hughes Medical Institute The Classical Association UC Berkeley UCSF University of California University of California in San Francisco Agilent BD Biosciences Bio-Rad Lonza New England Biolabs Promega Sigma-Aldrich Takara Bio Thermo Fisher Scientific Coherent OBIS 405 Coherent OBIS 488 diode Andor EMCCD Andor iXon Andor Ixon Ultra Andor sCMOS Andor Zyla 4.2 EMCCD Hamamatsu sCMOS Olympus CCD 100x/1.4 NA oil Chroma ET525/50 Chroma ET595/50

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