Microscope • 10 papers
High-speed atomic force microscopy is a technique that allows real-time observation of biomolecules and biological phenomena reconstituted on a substrate. Here, we present a protocol...
Background Chronic respiratory diseases represent a large group of non-communicable diseases that are a leading cause of mortality and morbidity globally. Many of the methods...
The endothelium is the gatekeeper of vessel health, and its dysfunction is pivotal in driving atherogenesis. Here, we present a protocol to replicate endothelial-macrophage crosstalk...
Gold nanoparticles (AuNPs) are increasingly used in applications across the biomedical domain, yet their long-term biodistribution and biocompatibility remain poorly understood. Conventional brightfield microscopy imaging...
Abstract Background Cardiomyocyte proteostasis and calcium homeostasis are critical for maintaining cardiac function, with their dysregulation contributing to cardiac hypertrophy and heart failure. The Tripartite...
The dysfunction of α and β cells in pancreatic islets can lead to diabetes. Many questions remain on the subcellular organization of islet cells during...
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this...
AbstractSynucleinopathies such as Parkinson's disease (PD) are hallmarked by α‐synuclein (α‐syn) pathology and neuroinflammation. This neuroinflammation involves activated microglia with increased secretion of interleukin‐1β (IL‐1β)....
Negative stain electron microscopy (EM) allows relatively simple and quick observation of macromolecules and macromolecular complexes through the use of contrast enhancing stain reagent. Although...
Abstract Purified mitochondrial ATP synthase has been shown to form Ca 2+ -activated, large conductance channel activity similar to that of mitochondrial megachannel (MMC) or...