Leica SP5

Leica SP5

Microscope • 358 papers

Imaging and quantitative analysis of integrin-dependent cell-matrix adhesions.

STAR protocols 2023 📊 1

Integrin-dependent cell-extracellular matrix adhesion is essential for wound healing, embryonic development, immunity, and tissue organization. Here, we present a protocol for the imaging and quantitative...

📋 1 💻 Code 💾 Data 🏷️ 1 RRID

Activation of GluN2D-containing NMDA receptors promotes development of axons and axon-carrying dendrites of cortical interneurons.

Cerebral cortex (New York, N.Y. : 1991) 2025 📊 4

Abstract GluN2D-containing NMDA receptors are expressed in early postnatal interneurons, but their role is enigmatic. We tested whether treatment with the GluN2C/D positive allosteric modulator...

🏷️ 22 RRIDs

Visualisation and quantification of intracellular interactions of Neisseria meningitidis and human α-actinin by confocal imaging.

Journal of visualized experiments : JoVE 2010

The Opc protein of Neisseria meningitidis (Nm, meningococcus) is a surface-expressed integral outer membrane protein, which can act as an adhesin and an effective invasin...

Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster.

Bio-protocol 2023

Adult muscle stem cells (MuSCs) show remarkable capability in repairing injured tissues. Studying MuSCs in suitable model organisms, which show strong homology with vertebrate counterparts,...

Genetically-encoded markers for confocal visualization of single dense core vesicles.

Communications biology 2025 📊 9

Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors, and bioamines. The analysis of single DCVs has largely been...

Cortical circuitry mediating inter-areal touch signal amplification.

bioRxiv : the preprint server for biology 2023 📊 2

Abstract Sensory cortical areas are often organized into topographic maps which represent the sensory epithelium 1,2 . Individual areas are richly interconnected 3 , in...

A multiwell plate approach to increase the sample throughput during tissue clearing.

Nature protocols 2025 📊 2

Tissue clearing, coupled with immunostaining, enables the transition from two-dimensional to three-dimensional pathology and has the potential to substantially improve data quality for biomedical diagnostics....

Development of a New Bead Movement-Based Computational Framework Shows that Bacterial Amyloid Curli Reduces Bead Mobility in Biofilms.

Journal of bacteriology 2020 📊 2

Biofilms exist in complex environments, including the intestinal tract, as a part of the gastrointestinal microbiota. The interaction of planktonic bacteria with biofilms can be...

Developing Analysis Protocols for Monitoring Intracellular Oxygenation Using Fluorescence Lifetime Imaging of Myoglobin-mCherry.

Methods in molecular biology (Clifton, N.J.) 2021 📊 2

Oxygen (O2) is a critical metabolite for cellular function as it fuels aerobic cellular metabolism; further, it is a known regulator of gene expression. Monitoring...

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The destruction of cytoplasmic skeleton leads to the change of nuclear structure and the looseness of lamin A submicroscopic network.

Heliyon 2024 📊 2

The interaction between lamin A and the cytoplasmic skeleton plays a key role in maintaining nuclear mechanical properties. However, the effect of destruction of the...

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