Darkfield Microscopy

Darkfield Microscopy

Technique • 80 papers

CHOP is dispensable for lens transparency in wild-type and connexin50 mutant mice.

Molecular vision 2019

PURPOSE: CCAAT/enhancer-binding homologous protein (CHOP), a transcription factor that has been implicated in differentiation, apoptosis, and autophagy, is greatly elevated in lenses with cataracts due...

🔥 High Impact

Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles.

Nature methods 2022 📊 87

AbstractLabel-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing...

⭐ Foundational

Classification and Segmentation of Nanoparticle Diffusion Trajectories in Cellular Micro Environments.

PloS one 2017 📊 123

Darkfield and confocal laser scanning microscopy both allow for a simultaneous observation of live cells and single nanoparticles. Accordingly, a characterization of nanoparticle uptake and...

⭐ Foundational

TISSUE CLEARING.

Nature reviews. Methods primers 2021 📊 163

Tissue clearing of gross anatomical samples was first described over a century ago and has only recently found widespread use in the field of microscopy....

3D ImagingConfocal Laser Scanning MicroscopyDarkfield MicroscopyDeconvolution MicroscopyExpansion MicroscopyFluorescence MicroscopyLight Sheet Fluorescence MicroscopyOptical Coherence TomographyOptical SectioningSecond Harmonic GenerationSpinning Disk Confocal MicroscopyTwo-Photon Excitation MicroscopyZ-Stack ImagingLaVision BioTec UltraMicroscope Light SheetUltraMicroscopeDrosophila melanogasterAiviaArivisFijiilastikImageJImarisinFormMetaMorphnapariStarDistATTO 647DAPIDiIGFPmCherryPropidium IodideSYTOWGAAdeno-Associated VirusAntigen RetrievalBrain OrganoidClear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hydrogelClear Unobstructed Brain Imaging Cocktails and Computational analysisClustered Regularly Interspaced Short Palindromic RepeatsCryosectioningEnhanced Fluorescence-Assisted Shotgun HistologyExpansion MicroscopyFixationGlutaraldehydeImmunofluorescenceImmunohistochemistryImmunolabeling-enabled Three-Dimensional Imaging of Solvent-Cleared OrgansImmunostainingOrganoidParaformaldehyde FixationPermeabilizationPolyethylene Glycol-Associated Solvent SystemRetinal OrganoidSequential Fluorescence In Situ HybridizationSpheroidStabilization to Harsh conditions via Intramolecular Epoxide Linkages to prevent DegradationTissue ClearingTransductionUltimate Three-Dimensional Imaging of Solvent-Cleared OrgansVibratomeLaVision BioTec UltraMicroscope Light SheetAiviaarivis Proarivis Vision4DFijiilastikImageJImarisinFormnapariStarDistMetaMorphBroad InstituteBroad Institute of Harvard University and MITHarvard UniversityInstitut für Schlaganfall- und DemenzforschungLudwig-Maximilians-Universität MünchenMassachusetts Institute of TechnologyMaximilians UniversityMITPicower Institute for Learning and MemoryRIKENThe UniversityUniversity and MITUniversity of TokyoYonsei UniversityThermo Fisher Scientific
⭐ Foundational

Interferometric Scattering Microscopy: Seeing Single Nanoparticles and Molecules via Rayleigh Scattering.

Nano letters 2019 📊 224

Fluorescence microscopy has been the workhorse for investigating optical phenomena at the nanometer scale but this approach confronts several fundamental limits. As a result, there...

Dimerization of GAS2 mediates crosslinking of microtubules and F-actin.

The EMBO journal 2025 📊 3

Abstract The spectraplakin family protein GAS2 was originally identified as a growth arrest-specific protein, and recent studies have revealed its involvement in multiple cellular processes....

💾 Data

Active Zone: Linking Resolution Levels of Microscopic Modalities.

Advances in neurobiology 2026

Abstract The presynaptic active zone (AZ) is a precisely organized nanoscale domain where synaptic vesicle exocytosis and neurotransmitter release are governed by tightly regulated protein...

Axonemal dynein contributions to flagellar beat types and waveforms.

bioRxiv : the preprint server for biology 2025

Eukaryotic flagella, or motile cilia, are iconic molecular machines whose beating drives cell propulsion and fluid transport across diverse organisms. Beat type and waveform are...

💻 Code 💾 Data

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