LSM 700

LSM 700

Microscope • 30 papers

⭐ Foundational

Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner.

eLife 2015 📊 137

Soluble karyopherins of the importin-β (impβ) family use RanGTP to transport cargos directionally through the nuclear pore complex (NPC). Whether impβ or RanGTP regulate the...

Confocal Laser Scanning MicroscopyDirect Stochastic Optical Reconstruction MicroscopyFluorescence MicroscopyFluorescence Recovery After PhotobleachingImmunofluorescence MicroscopyStochastic Optical Reconstruction MicroscopyLSM 700TiZeiss LSM 700Zeiss LSM 700 ConfocalEscherichia coliFijiImageJMATLABPythonBFPCy3Cy5DiDEdUFITCGFPmCherrymEos2YFPBlockingCell CultureFixationImmunofluorescenceKnockdownLipofectionParaformaldehyde FixationPermeabilizationTransfectionEmbryonic Stem CellHeLa (Henrietta Lacks)Zeiss LSM 700 ConfocalImageJMATLABPythonBirkbeck, University of LondonÉcole Polytechnique Fédérale de LausanneHoward Hughes Medical InstituteQB3University College LondonUniversity College London and Birkbeck CollegeUniversity of CaliforniaAbcamInvitrogenMatTekQiagenRocheSigma-AldrichDonkeyMouseRabbitCoherent SapphireCoherent SapphiresdiodeAndor EMCCDAndor iXonAndor iXon 897Andor Ixon UltraEMCCD100x/1.49 NAOlympus 60x/1.49 NA
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⭐ Foundational

Visual Input to the Drosophila Central Complex by Developmentally and Functionally Distinct Neuronal Populations.

Current biology : CB 2017 📊 137

The Drosophila central brain consists of stereotyped neural lineages, developmental-structural units of macrocircuitry formed by the sibling neurons of single progenitors called neuroblasts. We demonstrate...

Calcium ImagingConfocal Laser Scanning MicroscopyOptical SectioningLSM 700Zeiss LSM 700 ConfocalDrosophila melanogasterSlideBookGCaMP6mGFPmCherryRFPFixationImmunohistochemistryImmunostainingMouse Embryonic FibroblastZeiss LSM 700 ConfocalImageJSlideBookUCLAUniversity of CaliforniaVector Laboratories
⭐ Foundational

Miro clusters regulate ER-mitochondria contact sites and link cristae organization to the mitochondrial transport machinery.

Nature communications 2019 📊 167

Abstract Mitochondrial Rho (Miro) GTPases localize to the outer mitochondrial membrane and are essential machinery for the regulated trafficking of mitochondria to defined subcellular locations....

Confocal Laser Scanning MicroscopyDirect Stochastic Optical Reconstruction MicroscopyFluorescence MicroscopyFluorescence Recovery After PhotobleachingStochastic Optical Reconstruction MicroscopyStructured Illumination MicroscopyTotal Internal Reflection Fluorescence MicroscopyElyraLSM 700Zeiss ElyraZeiss Elyra 7 Super-ResolutionZeiss LSM 700Zeiss LSM 700 ConfocalSaccharomyces cerevisiaeExcelFijiGraphPad PrismImageJIN CellPythonDAPIDsRedEGFPGFPBlockingCell CultureFixationImmunostainingKnockoutLipofectionParaformaldehyde FixationTransfectionHeLa (Henrietta Lacks)Mouse Embryonic FibroblastPrimary FibroblastsZeiss Elyra 7 Super-ResolutionZeiss LSM 700 ConfocalImageJIN CellExcelGraphPad PrismPythonBirkbeck, University of LondonDepartment of Biological SciencesKing's College LondonLondon Centre for NanotechnologyMRC Laboratory for Molecular Cell BiologyTata Institute of Fundamental ResearchUCLUniversity College LondonAddgeneGibcoLonzaSigma-Aldrich100x/1.46 NA oil
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⭐ Foundational

Lgr5+ cells regenerate hair cells via proliferation and direct transdifferentiation in damaged neonatal mouse utricle.

Nature communications 2015 📊 166

Recruitment of endogenous progenitors is critical during tissue repair. The inner ear utricle requires mechanosensory hair cells (HCs) to detect linear acceleration. After damage, non-mammalian...

Confocal Laser Scanning MicroscopyZ-Stack ImagingAxio ObserverCSU-X1LSM 700Observer 7Zeiss Axio ObserverZeiss LSM 700 ConfocalMetaMorphAlexa Fluor 488Alexa Fluor 594Cy5DAPIEdUEGFPFITCPhalloidintdTomatoTRITCBlockingFixationImmunohistochemistryIn Situ HybridizationRNAscopeTerminal Deoxynucleotidyl Transferase dUTP Nick End LabelingTransductionZeiss LSM 700 ConfocalFijiImageJMetaMorphVolocityMetaMorphExcelGraphPad PrismKey Laboratory for Developmental Genes and Human DiseaseMEMPHIS: Modèles et méthodes pour les problèmes multiphysiques et interactionsNantong UniversityNational Institutes of Healths Research HospitalSoutheast UniversityStanford UniversityUniversity School of MedicineAbcamBD BiosciencesDakoInvitrogenLife TechnologiesMatTekMilliporeQiagenSanta Cruz BiotechnologySigma-Aldrich
⭐ Foundational

Descending control of swim posture by a midbrain nucleus in zebrafish.

Neuron 2014 📊 189

The reticular formation in the brainstem controls motor output via axonal projections to the hindbrain and spinal cord. It remains unclear how individual groups of...

Calcium ImagingConfocal Laser Scanning MicroscopyOptical SectioningOptogeneticsTwo-Photon Excitation MicroscopyLSM 700LSM 780Zeiss LSM 700 ConfocalZeiss LSM 780 ConfocalCaenorhabditis elegansScanImageZENGCaMP6GCaMP6sGFPKaedemCherryTexas RedTRITCCryosectioningElectroporationFixationImmunohistochemistryParaformaldehyde FixationZeiss LSM 700 ConfocalZeiss LSM 780 ConfocalFijiPythonZENScanImageZENIgor ProPythonMax PlanckMax Planck InstituteMax Planck Institute of NeurobiologyUniversity of California at San FranciscoAddgeneGeneTexInvitrogenMilliporeGoatMouseCoherent Chameleon UltraTi:Sapphire
⭐ Foundational

Deep learning-based predictive identification of neural stem cell differentiation.

Nature communications 2021 📊 203

AbstractThe differentiation of neural stem cells (NSCs) into neurons is proposed to be critical in devising potential cell-based therapeutic strategies for central nervous system (CNS)...

Confocal Laser Scanning MicroscopyImmunofluorescence MicroscopyTransmission Electron MicroscopyLSM 700Zeiss LSM 700 ConfocalZENAlexa Fluor 488APCDAPIBlockingFixationImmunofluorescenceImmunostainingNeural Stem CellsPrimary AstrocytesPrimary FibroblastsPrimary OligodendrocytesZeiss LSM 700 ConfocalImageJZENExcelGraphPad PrismPythonKey Laboratory of Spine and Spinal Cord Injury Repair and RegenerationMinistry of EducationTongji HospitalTongji UniversityUniversity School of MedicineAbcamBD BiosciencesGibcoMerckMilliporeRocheSigma-AldrichTakara BioDonkeyGoatMouseRabbit
⭐ Foundational

A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation.

Journal of extracellular vesicles 2022 📊 230

AbstractNanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell‐derived extracellular vesicles (EVs). Here we demonstrate that...

Confocal Laser Scanning MicroscopyDirect Stochastic Optical Reconstruction MicroscopyStochastic Optical Reconstruction MicroscopySuper-Resolution MicroscopyTransmission Electron MicroscopyAxio ObserverGlaciosLSM 700Observer 7TiZeiss Lightsheet Z.1 Light SheetZeiss LSM 700 ConfocalHomo sapiensNIS-ElementsAlexa Fluor 488Alexa Fluor 647APCATTO 488BODIPYCalceinCalcein-AMCy3Cy7FITCCell CultureFixationGlutaraldehydeHematoxylin and EosinImmunohistochemistryPrimary FibroblastsPrimary KeratinocytesPrimary Mesenchymal Stem CellsPrimary MonocytesPrimary Natural Killer CellsPrimary T CellsZeiss Lightsheet Z.1 Light SheetZeiss LSM 700 ConfocalImageJSerialEMNIS-ElementsGraphPad PrismRLodron UniversityMedical UniversityMedical University of ViennaPomeranian Medical UniversityUniversity of ViennaUniversity SalzburgAbcamAgilentBD BiosciencesBiotiumDakoeBioscienceGibcoibidiLife TechnologiesLonzaMerckMilliporePromegaQiagenR&D SystemsRocheSigma-AldrichThermo Fisher ScientificVWRThermo Fisher Falcon III
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⭐ Foundational

Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells.

Nature communications 2019 📊 212

AbstractThe orchestration of intercellular communication is essential for multicellular organisms. One mechanism by which cells communicate is through long, actin-rich membranous protrusions called tunneling nanotubes...

Confocal Laser Scanning MicroscopyCorrelative Light and Electron MicroscopyCryo-Electron MicroscopyCryo-Electron TomographyElectron TomographyFocused Ion Beam Scanning Electron MicroscopyImmunofluorescence MicroscopyLive Cell ImagingScanning Electron MicroscopyWidefield Fluorescence MicroscopyZ-Stack ImagingAxiovertLSM 700TecnaiTiZeiss LSM 700Zeiss LSM 700 ConfocalAxioVisionZENAlexa Fluor 488DAPIDiDGFPmCherryMitoTrackerPhalloidinRhodamineWGACell CultureFixationGlutaraldehydeImmunofluorescenceLipofectionLive ImagingParaformaldehyde FixationPermeabilizationTransfectionHuman Neuroblastoma SH-SY5YZeiss LSM 700 ConfocalAmiraDigital MicrographIcyImageJIMODSerialEMAxioVisionZENInstitut PasteurState UniversityThe Classical AssociationAbcamCorningGibcoibidiInvitrogenMatTekSigma-AldrichThermo Fisher ScientificGoatMouseRabbitNikon CCDNikon 60x/1.4 NA
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⭐ Foundational

Dopamine neurons projecting to the posterior striatum form an anatomically distinct subclass.

eLife 2015 📊 304

Combining rabies-virus tracing, optical clearing (CLARITY), and whole-brain light-sheet imaging, we mapped the monosynaptic inputs to midbrain dopamine neurons projecting to different targets (different parts...

Confocal Laser Scanning MicroscopyOptical SectioningLSM 700Zeiss LSM 700Zeiss LSM 700 ConfocalilastikMATLABBFPEGFPmTagBFPtdTomatoAdeno-Associated VirusClear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hydrogelFixationImmunohistochemistryParaformaldehyde FixationTissue ClearingZeiss LSM 700 ConfocalilastikMATLABCold Spring Harbor LaboratoryHarvard UniversityAbcamSigma-AldrichThermo Fisher ScientificChickenRabbitsCMOS
📋 18 💾 Data
⭐ Foundational

Thalamic nuclei convey diverse contextual information to layer 1 of visual cortex.

Nature neuroscience 2016 📊 342

Sensory perception depends on the context in which a stimulus occurs. Prevailing models emphasize cortical feedback as the source of contextual modulation. However, higher order...

Calcium ImagingConfocal Laser Scanning MicroscopyTwo-Photon Excitation MicroscopyLSM 700Zeiss LSM 700 ConfocalScanImageDAPIDiIEGFPGCaMPGCaMP6GCaMP6fGCaMP6mGFPtdTomatoAdeno-Associated VirusVibratomeZeiss LSM 700 ConfocalinFormScanImageLabVIEWMATLABUniversity College LondonUniversity of BaselInvitrogenLife TechnologiesSigma-AldrichThermo Fisher ScientificVector LaboratoriesSpectra-Physics Mai TaiSpectra-Physics MaiTai DeepSeeTi:SapphireEdmund Optics CCD

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