SP5 Microscopy Papers – Page 3

⭐ Foundational

Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression.

Nature communications 2018 📊 130

AbstractFractional killing is the main cause of tumour resistance to chemotherapy. This phenomenon is observed even in genetically identical cancer cells in homogeneous microenvironments. To...

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⭐ Foundational

Mycobacterium tuberculosis replicates within necrotic human macrophages.

The Journal of cell biology 2017 📊 134

Mycobacterium tuberculosis modulation of macrophage cell death is a well-documented phenomenon, but its role during bacterial replication is less characterized. In this study, we investigate...

Confocal Laser Scanning Microscopy Correlative Light and Electron Microscopy Immunofluorescence Microscopy Live Cell Imaging Scanning Electron Microscopy Serial Block-Face Scanning Electron Microscopy Transmission Electron Microscopy JEM-1400 JEOL JEM-1400 Electron Microscope Leica SP5 Confocal SP5 Tecnai Amira Digital Micrograph Fiji ImageJ LAS X R Alexa Fluor 488 Alexa Fluor 546 APC BV421 DAPI EGFP GFP Blocking Cell Culture Fixation Glutaraldehyde Immunofluorescence Live Imaging Paraformaldehyde Fixation Permeabilization Ultramicrotome Embryonic Stem Cell Primary Macrophages Primary Monocytes JEOL JEM-1400 Electron Microscope Leica SP5 Confocal Amira Digital Micrograph Fiji ImageJ LAS X R Francis Crick Institute University of Oslo Cell Signaling Technology Dako eBioscience Gibco Jackson ImmunoResearch Lonza MatTek Promega Sigma-Aldrich Thermo Fisher Scientific Mouse Rabbit Leica HyD 63x/1.4 NA

⭐ Foundational

Cell-to-cell transfer of HIV-1 via virological synapses leads to endosomal virion maturation that activates viral membrane fusion.

Cell host & microbe 2011 📊 135

HIV-1 can infect T cells by cell-free virus or by direct virion transfer between cells through cell contact-induced structures called virological synapses (VS). During VS-mediated...

⭐ Foundational

An autocrine signaling circuit in hepatic stellate cells underlies advanced fibrosis in nonalcoholic steatohepatitis.

Science translational medicine 2023 📊 136

Advanced hepatic fibrosis, driven by the activation of hepatic stellate cells (HSCs), affects millions worldwide and is the strongest predictor of mortality in nonalcoholic steatohepatitis...

3D Imaging Confocal Laser Scanning Microscopy Immunofluorescence Microscopy Light Sheet Fluorescence Microscopy Leica DMi8 Leica SP5 Confocal SP5 Imaris LAS X Python Propidium Iodide Cell Culture Clustered Regularly Interspaced Short Palindromic Repeats Fixation Hematoxylin and Eosin Immunofluorescence Immunohistochemistry Immunolabeling-enabled Three-Dimensional Imaging of Solvent-Cleared Organs Knockdown Lentiviral Microtome Monolayer Optimal Cutting Temperature Embedding Paraformaldehyde Fixation Tissue Clearing Transduction Hematopoietic Stem Cells Primary Endothelial Cells Leica DMi8 Leica SP5 Confocal Imaris LAS X Python Howard Hughes Medical Institute Icahn School of Medicine at Mount Sinai University of California University of Edinburgh University School of Medicine Weizmann Institute Weizmann Institute of Science Yale University Abcam Addgene Agilent Bio-Rad Cell Signaling Technology Millipore R&D Systems Sigma-Aldrich Thermo Fisher Scientific

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⭐ Foundational

Size-dependent effects of gold nanoparticles uptake on maturation and antitumor functions of human dendritic cells in vitro.

PloS one 2014 📊 137

Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects....

⭐ Foundational

Candida albicans internalization by host cells is mediated by a clathrin-dependent mechanism.

Cellular microbiology 2009 📊 140

Candida albicans is a major cause of oropharyngeal, vulvovaginal and haematogenously disseminated candidiasis. Endocytosis of C. albicans hyphae by host cells is a prerequisite for...

⭐ Foundational

Micropilot: automation of fluorescence microscopy-based imaging for systems biology.

Nature methods 2011 📊 144

Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects...

Fluorescence Microscopy Fluorescence Recovery After Photobleaching Live Cell Imaging SP5 CellProfiler LAS X EGFP Hoechst 33342 Knockdown HeLa (Henrietta Lacks)SP5 CellProfiler LAS X European Molecular Biology Laboratory PerkinElmer

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⭐ Foundational

A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout.

eLife 2016 📊 147

Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal...

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⭐ Foundational

Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells.

PloS one 2011 📊 161

High-grade gliomas (World Health Organization grade III anaplastic astrocytoma and grade IV glioblastoma multiforme), the most prevalent primary malignant brain tumors, display a cellular hierarchy...

⭐ Foundational

The evolutionary dynamics of extrachromosomal DNA in human cancers.

Nature genetics 2022 📊 173

AbstractOncogene amplification on extrachromosomal DNA (ecDNA) is a common event, driving aggressive tumor growth, drug resistance and shorter survival. Currently, the impact of nonchromosomal oncogene...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Live Cell Imaging Z-Stack Imaging BX63 Leica SP5 Confocal Leica SP8 Confocal LSM 880 Olympus BX63 SP5 SP8 Zeiss LSM 880 Confocal Fiji ImageJ LAS X Alexa Fluor 488 DAPI EGFP GFP Blocking Cell Culture Clustered Regularly Interspaced Short Palindromic Repeats Electroporation Fixation Fluorescence In Situ Hybridization Immunofluorescence Lentiviral Live Imaging Paraformaldehyde Fixation Permeabilization Transfection Human Prostate Cancer PC-3 Leica SP5 Confocal Leica SP8 Confocal Olympus BX63 Zeiss LSM 880 Confocal ImageJ LAS X Berlin Institute of Health California Institute for Regenerative Medicine Center of Excellence Experimental and Clinical Research Center Francis Crick Institute German Cancer Consortium and German Cancer Research Center Howard Hughes Medical Institute Janelia Research Campus Mary University Max Planck Max Planck Institute Max Planck Institute for Evolutionary Biology Princeton University Queen Mary University of London s Medical Center sen University St. Anna Children's Cancer Research Institute Stanford University Sun Yat-sen University The Classical Association The State Key Laboratory of Biocontrol UCSD Universitätsmedizin Berlin University College London University College London Cancer Institute University College London Hospitals University of California University of California San Diego University of California San Diego Light Microscopy Core Facility University of London University of Texas Southwestern Medical Center University School of Medicine Addgene Bio-Rad Corning Dako Gibco Promega Qiagen Thermo Fisher Scientific Photometrics CoolSnap

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