Community OpenSPIM Light Sheet

Community OpenSPIM Light Sheet

Microscope • 8 papers

⭐ Foundational

The ImageJ ecosystem: An open platform for biomedical image analysis.

Molecular reproduction and development 2015 📊 2,519

SUMMARYTechnology in microscopy advances rapidly, enabling increasingly affordable, faster, and more precise quantitative biomedical imaging, which necessitates correspondingly more‐advanced image processing and analysis techniques. A...

Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.) 2024

The recent significant progress in developmental bio-imaging of live multicellular organisms has been greatly facilitated by the development of light sheet fluorescence microscopy (LSFM). Both...

A dual-modality optical coherence tomography and selective plane illumination microscopy system for mouse embryonic imaging.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference 2017

Both optical coherence tomography (OCT) and selective plane illumination microscopy (SPIM) are frequently used in mouse embryonic research for high-resolution three-dimensional imaging. Each of these...

🔥 High Impact

Extending resolution within a single imaging frame.

Nature communications 2022 📊 71

Abstract The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have...

Airyscan MicroscopyAtomic Force MicroscopyConfocal Laser Scanning MicroscopyFluorescence MicroscopyImmunofluorescence MicroscopyLight Sheet Fluorescence MicroscopyLive Cell ImagingSingle-Molecule Localization MicroscopyStimulated Emission Depletion MicroscopyStructured Illumination MicroscopySuper-Resolution MicroscopySuper-Resolution Optical Fluctuation ImagingTotal Internal Reflection Fluorescence MicroscopyZ-Stack ImagingAbberior STEDYCON Super-ResolutionCommunity OpenSPIM Light SheetOlympus FV1000Olympus FV1000 ConfocalOpenSPIMZeiss AxiovertZeiss Celldiscoverer 7Zeiss Elyra 7 Super-ResolutionZeiss LSM 880 ConfocalArabidopsis thalianaEscherichia coliZENZEN BlackAlexa Fluor 488Alexa Fluor 568APCATTO 488ATTO 647ATTO 655DAPIFITCHoechst 33342mEmeraldMitoTrackerPhalloidinRFPYFPClustered Regularly Interspaced Short Palindromic RepeatsFixationImmunofluorescenceLive ImagingEmbryonic Stem CellAbberior STEDYCON Super-ResolutionCommunity OpenSPIM Light SheetOlympus FV1000 ConfocalZeiss Celldiscoverer 7Zeiss Elyra 7 Super-ResolutionZeiss LSM 880 ConfocalIcyTrackMateZENZEN BlackMATLABRColorado State UniversityEuropean Molecular Biology LaboratoryModelica AssociationState UniversityUniversidad Autónoma del Estado de MorelosUniversidad Nacional Autónoma de MéxicoUniversity of Rome Tor VergataAbcamAddgeneCytivaInvitrogenNew England BiolabsSanta Cruz BiotechnologyGoatMouseRabbitAndor CCDAndor iXonAndor iXon 897CCDEMCCDHamamatsu Orca Flash 4.0Hamamatsu ORCA FusionONI sCMOSsCMOSZeiss Airyscan100x/1.4 NA100x/1.46 NA100x/1.47 NA oilZeiss 60x/1.4 NA oilZeiss Plan-Apochromat 63x630/92 bandpassdichroic 561
💾 Data 🏷️ 5 RRIDs
🔥 High Impact

Calcium dynamics in root cells of Arabidopsis thaliana visualized with selective plane illumination microscopy.

PloS one 2013 📊 65

Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or...

🔥 High Impact

Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods.

Breast cancer research : BCR 2016 📊 93

BACKGROUND: High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and...

🔥 High Impact

Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene.

Scientific reports 2016 📊 95

AbstractWhole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse...

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