Lightsheet Z.1 Microscopy Papers

⭐ Foundational

Fast and accurate sCMOS noise correction for fluorescence microscopy.

Nature communications 2020 📊 125

AbstractThe rapid development of scientific CMOS (sCMOS) technology has greatly advanced optical microscopy for biomedical research with superior sensitivity, resolution, field-of-view, and frame rates. However,...

⭐ Foundational

Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy.

Nature communications 2017 📊 278

AbstractSubunit vaccines have been investigated in over 1000 clinical trials of cancer immunotherapy, but have shown limited efficacy. Nanovaccines may improve efficacy but have rarely...

3D Imaging Atomic Force Microscopy Confocal Laser Scanning Microscopy Deconvolution Microscopy Fluorescence Microscopy Light Sheet Fluorescence Microscopy Structured Illumination Microscopy Super-Resolution Microscopy Transmission Electron Microscopy Leica SP8 Leica SP8 Confocal Lightsheet Z.1 LSM 780 SP8 Ti Zeiss Lightsheet Z.1 Light Sheet Zeiss LSM 780 Zeiss LSM 780 Confocal GraphPad Prism LAS X APC APC-Cy7 BV421 Cy5 Cy5.5 Cy7 DAPI FITC Hoechst 33342 LysoTracker LysoTracker Green LysoTracker Red PE PE-Cy5 PerCP Cell Culture Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hydrogel Co-culture Fixation Lipofection Paraformaldehyde Fixation Passive CLARITY Technique Permeabilization Tissue Clearing Transfection Mouse Macrophage RAW 264.7 Mouse Melanoma B16 Primary Macrophages Primary Natural Killer Cells Primary T Cells Leica SP8 Confocal Zeiss Lightsheet Z.1 Light Sheet Zeiss LSM 780 Confocal LAS X GraphPad Prism China Pharmaceutical University Ministry of Defence National Institutes of Health NIH Pharmaceutical University State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics The Classical Association University of Southern California Vaccine Research Center Xiamen University BD Biosciences BioLegend eBioscience Invitrogen Life Technologies Sigma-Aldrich Thermo Fisher Scientific

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🔥 High Impact

Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina.

The Journal of cell biology 2016 📊 80

The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate...

Confocal Laser Scanning Microscopy Immunofluorescence Microscopy Light Sheet Fluorescence Microscopy Live Cell Imaging Spinning Disk Confocal Microscopy Z-Stack Imaging CSU-X1 Lightsheet Z.1 LSM 510 LSM 780 Yokogawa CSU-X1 Spinning Disk Zeiss Lightsheet Z.1 Light Sheet Zeiss LSM 510 Confocal Zeiss LSM 780 Confocal Homo sapiens Excel Fiji ImageJ ZEN Acridine Orange Alexa Fluor 488 CFP DAPI DRAQ5 DsRed EGFP GFP mKate2 Phalloidin RFP tdTomato YFP Fixation Immunofluorescence Immunostaining Knockdown Live Imaging Permeabilization Whole Mount Yokogawa CSU-X1 Spinning Disk Zeiss Lightsheet Z.1 Light Sheet Zeiss LSM 510 Confocal Zeiss LSM 780 Confocal Fiji ImageJ Excel Instituto de Biofísica Carlos Chagas Filho Max Planck Max Planck Institute Max Planck Institute of Molecular Cell Biology and Genetics Abcam Addgene Enzo Life Sciences MatTek Merck New England Biolabs Roche Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific Andor sCMOS sCMOS Olympus UPLSAPO 60x Zeiss Plan-Apochromat 20x

💾 Data 🏷️ 4 RRIDs

⭐ Foundational

Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator.

Neuron 2020 📊 127

Methods for one-photon fluorescent imaging of calcium dynamics can capture the activity of hundreds of neurons across large fields of view at a low equipment...

Calcium Imaging Confocal Laser Scanning Microscopy Spinning Disk Confocal Microscopy Lightsheet Z.1 Ti Yokogawa CSU-W1 Spinning Disk Zeiss Lightsheet Z.1 Light Sheet NIS-Elements EdU GCaMP GCaMP6 GCaMP6f GCaMP7f GFP mCherry miRFP mScarlet RFP Adeno-Associated Virus Electroporation Fixation Permeabilization Transduction Transfection Vibratome Primary Neurons Yokogawa CSU-W1 Spinning Disk Zeiss Lightsheet Z.1 Light Sheet ImageJ NIS-Elements MATLAB Python Baylor College of Medicine Boston Children's Hospital Boston University Department of Neurobiology and Pittsburgh Institute for Neurodegenerative Diseases Harvard Medical School Howard Hughes Medical Institute Janelia Research Campus KAIST Institute for Health Science and Technology Massachusetts Institute of Technology MIT MIT McGovern Institute for Brain Research Modelica Association Rice University s Hospital Salk Institute Salk Institute for Biological Studies The Classical Association The Picower Institute for Learning and Memory University of California University of Pittsburgh University of Pittsburgh School of Medicine Vanderbilt University Westlake University Addgene Invitrogen Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific Mouse Rabbit Rat Andor sCMOS Andor Zyla5.5 Hamamatsu Orca Flash 4.0 Hamamatsu ORCA-flash4.0 sCMOS 20x/1.0 NA water N Plan 10x Zeiss 10x/0.2 NA 470/24 bandpass 525/50 bandpass Andor 525/50 bandpass Lumencor 470/24 bandpass Semrock FF493/574

💻 Code 💾 Data

⭐ Foundational

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb.

eLife 2018 📊 142

During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms...

💻 Code 💾 Data

⭐ Foundational

Rapid image deconvolution and multiview fusion for optical microscopy.

Nature biotechnology 2020 📊 155

The contrast and resolution of images obtained with optical microscopes can be improved by deconvolution and computational fusion of multiple views of the same sample,...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Fluorescence Microscopy Lattice Light Sheet Microscopy Light Sheet Fluorescence Microscopy Structured Illumination Microscopy Widefield Fluorescence Microscopy diSPIM iSPIM Leica SP8 Leica SP8 Confocal Lightsheet Z.1 SP8 Zeiss Lightsheet 7 Light Sheet Zeiss Lightsheet Z.1 Zeiss Lightsheet Z.1 Light Sheet Caenorhabditis elegans Drosophila melanogaster Escherichia coli MicroManager ZEN Alexa Fluor 488 Alexa Fluor 555 DAPI EdU EGFP GFP mCherry mEmerald mRFP Phalloidin RFP TagRFP TagRFP-T tdTomato Blocking Fixation Glutaraldehyde Immunolabeling-enabled Three-Dimensional Imaging of Solvent-Cleared Organs Permeabilization Tissue Clearing Transfection Embryonic Stem Cell Human Osteosarcoma U2OS Human T-cell Leukemia Jurkat Primary T Cells Leica SP8 Confocal Zeiss Lightsheet 7 Light Sheet Zeiss Lightsheet Z.1 Light Sheet Huygens ImageJ Imaris MicroManager ZEN MATLAB Python Centre Thucydide - Analyse et Recherche en Relations Internationales Eunice Kennedy Shriver National Institute of Child Health and Human Development Harvard Medical School Ministry of Defence Modelica Association National Institute of Allergy and Infectious Diseases National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institute of Biomedical Imaging and Bioengineering National Institute of Mental Health National Institutes of Health NIH State Key Laboratory of Modern Optical Instrumentation UConn Health University of Chicago University of Maryland University of Puerto Rico System University School of Medicine Yale University Zhejiang University Abcam Addgene Corning Invitrogen MatTek Sigma-Aldrich Thermo Fisher Scientific VWR Donkey Goat Mouse Rabbit Coherent OBIS sources diode Hamamatsu Orca Flash 4.0 Leica HyD PCO sCMOS pco.edge 4.2 Photometrics EMCCD Photometrics evolve sCMOS 20x/1.0 NA 40x/0.8 NA water Zeiss 10x/0.2 NA dry Chroma ET470/40x Photometrics ET525/50m

💻 Code 💾 Data

⭐ Foundational

A crystal-clear zebrafish for in vivo imaging.

Scientific reports 2016 📊 147

The larval zebrafish (Danio rerio) is an excellent vertebrate model for in vivo imaging of biological phenomena at subcellular, cellular and systems levels. However, the...

⭐ Foundational

Efficient Bayesian-based multiview deconvolution.

Nature methods 2014 📊 230

Light-sheet fluorescence microscopy is able to image large specimens with high resolution by capturing the samples from multiple angles. Multiview deconvolution can substantially improve the...

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