LSM 880 Microscopy Papers – Page 2

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The evolutionary dynamics of extrachromosomal DNA in human cancers.

Nature genetics 2022 📊 173

AbstractOncogene amplification on extrachromosomal DNA (ecDNA) is a common event, driving aggressive tumor growth, drug resistance and shorter survival. Currently, the impact of nonchromosomal oncogene...

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⭐ Foundational

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture.

The Journal of cell biology 2018 📊 175

The two Condensin complexes in human cells are essential for mitotic chromosome structure. We used homozygous genome editing to fluorescently tag Condensin I and II...

Airyscan Microscopy Confocal Laser Scanning Microscopy Fluorescence Correlation Spectroscopy Fluorescence Recovery After Photobleaching Live Cell Imaging Reversible Saturable Optical Fluorescence Transitions Microscopy Stimulated Emission Depletion Microscopy Super-Resolution Microscopy Z-Stack Imaging Abberior Facility Line Super-Resolution Axio Observer IX83 LSM 780 LSM 880 Olympus IX83 Zeiss LSM 780 Confocal Zeiss LSM 880 Confocal Xenopus laevis Imspector ZEN Alexa Fluor 488 Alexa Fluor 594 GFP Hoechst 33342 mEGFP Blocking Cell Culture Clustered Regularly Interspaced Short Palindromic Repeats Fixation Immunostaining Live Imaging Paraformaldehyde Fixation Permeabilization Transfection HeLa (Henrietta Lacks)Abberior Facility Line Super-Resolution Olympus IX83 Zeiss LSM 780 Confocal Zeiss LSM 880 Confocal Fiji HALO ImageJ Imaris ThunderSTORM Imspector ZEN Excel MATLAB R EMBL European Molecular Biology Laboratory Abcam Bio-Rad Merck Promega Roche Sigma-Aldrich Thermo Fisher Scientific Chicken Goat Rabbit APD GaAsP Zeiss Airyscan Olympus 100x/1.4 NA oil Zeiss C-Apochromat 40x

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⭐ Foundational

DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.

Cell 2017 📊 215

Eukaryotic cells store their chromosomes in a single nucleus. This is important to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei) are prone...

Atomic Force Microscopy Confocal Laser Scanning Microscopy Deconvolution Microscopy Immunofluorescence Microscopy Live Cell Imaging Optical Sectioning LSM 780 LSM 880 Ti Zeiss LSM 780 Confocal Escherichia coli MetaMorph ZEN Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 633 EGFP GFP Hoechst 33342 mCherry mEGFP TagBFP TRITC Adenoviral Blocking Cell Culture Fixation Immunofluorescence Knockdown Lentiviral Live Imaging Permeabilization Transduction Transfection HeLa (Henrietta Lacks)Human Embryonic Kidney 293T Human Embryonic Kidney Lenti-X 293T Zeiss LSM 780 Confocal Imaris UCSF Chimera MetaMorph ZEN R Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 1030 Vienna, Austria.Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 1030 Vienna, Austria. Electronic address: daniel.gerlich@imba.oeaw.ac.at.Institute of Science and Technology Austria (IST Austria), 3400 Klosterneuburg, Austria.Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 1030 Vienna, Austria.Abcam Addgene BD Biosciences Bio-Rad Gibco ibidi Invitrogen Life Technologies Millipore New England Biolabs Roche Santa Cruz Biotechnology Sigma-Aldrich Takara Bio Thermo Fisher Scientific VWR Goat Mouse Rabbit

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⭐ Foundational

Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites.

eLife 2015 📊 292

While the dynamin GTPase Drp1 plays a critical role during mitochondrial fission, mechanisms controlling its recruitment to fission sites are unclear. A current assumption is...

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⭐ Foundational

Machine learning analysis of whole mouse brain vasculature.

Nature methods 2020 📊 308

Tissue clearing methods enable the imaging of biological specimens without sectioning. However, reliable and scalable analysis of large imaging datasets in three dimensions remains a...

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⭐ Foundational

Tissue clearing of both hard and soft tissue organs with the PEGASOS method.

Cell research 2018 📊 331

Tissue clearing technique enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. Current tissue clearing methods are restricted by limited...

Confocal Laser Scanning Microscopy Deconvolution Microscopy Fluorescence Microscopy Immunofluorescence Microscopy Light Sheet Fluorescence Microscopy Optical Sectioning Second Harmonic Generation Two-Photon Excitation Microscopy Z-Stack Imaging Leica SP5 Confocal Leica SP8 Confocal LSM 780 LSM 880 SP5 SP8 Zeiss LSM 780 Confocal Zeiss LSM 880 Confocal AutoQuant Excel Fiji GraphPad Prism ImageJ Imaris LAS X Alexa Fluor 488 EdU EGFP FITC GFP tdTomato YFP Blocking Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hydrogel Immunofluorescence Immunolabeling-enabled Three-Dimensional Imaging of Solvent-Cleared Organs Paraformaldehyde Fixation Three-Dimensional Imaging of Solvent-Cleared Organs Tissue Clearing Ultimate Three-Dimensional Imaging of Solvent-Cleared Organs Whole Mount Primary Astrocytes Primary Endothelial Cells Leica SP5 Confocal Leica SP8 Confocal Zeiss LSM 780 Confocal Zeiss LSM 880 Confocal AutoQuant Imaris LAS X Excel GraphPad Prism Chinese Academy of Sciences M University National Clinical Research Center Shanghai Institute of Biochemistry and Cell Biology Sichuan University State Key Laboratory of Cell Biology State Key Laboratory of Oral Diseases Texas A&M University University of Chinese Academy of Sciences UT Southwestern Medical Center West China Hospital Abcam Sigma-Aldrich Thermo Fisher Scientific VWR Goat Mouse Rabbit Coherent GaAsP Leica HyD PMT 1x/0.25 NA 25x/0.8 NA 32x/0.85 NA

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⭐ Foundational

A simple method for quantitating confocal fluorescent images.

Biochemistry and biophysics reports 2021 📊 366

Western blotting (WB), enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FC) have long been used to assess and quantitate relative protein expression in cultured cells...

⭐ Foundational

Phase separation of YAP reorganizes genome topology for long-term YAP target gene expression.

Nature cell biology 2019 📊 378

Yes-associated protein (YAP) is a transcriptional co-activator that regulates cell proliferation and survival by binding to a select set of enhancers for target gene activation....

Airyscan Microscopy Confocal Laser Scanning Microscopy Fluorescence Recovery After Photobleaching Immunofluorescence Microscopy Lattice Light Sheet Microscopy Light Sheet Fluorescence Microscopy Live Cell Imaging Photoactivated Localization Microscopy Z-Stack Imaging DMi8 Leica DMi8 Leica SP8 Confocal LSM 780 LSM 880 SP8 Zeiss LSM 780 Confocal Zeiss LSM 880 Confocal Escherichia coli ZEN Click-iT EdU EGFP GFP Hoechst 33342 JF549 mCherry Phalloidin RFP Blocking Cell Culture Clustered Regularly Interspaced Short Palindromic Repeats Fixation Immunofluorescence Knockdown Lipofection Live Imaging Paraformaldehyde Fixation Permeabilization Transfection Vibratome Human Embryonic Kidney 293 Human Embryonic Kidney 293T Human Osteosarcoma U2OS Leica DMi8 Leica SP8 Confocal Zeiss LSM 780 Confocal Zeiss LSM 880 Confocal ImageJ Imaris ZEN Eunice Kennedy Shriver National Institute for Child Health and Human Development HHMI Howard Hughes Medical Institute Instituto de Lingüística Janelia Research Campus National Institutes of Health The Classical Association University of California Abcam Addgene Agilent BD Biosciences Cell Signaling Technology Corning Gibco Invitrogen Millipore New England Biolabs Qiagen Sigma-Aldrich Leica HyD Zeiss Airyscan Zeiss Plan-Apochromat 63x

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⭐ Foundational

mito-QC illuminates mitophagy and mitochondrial architecture in vivo.

The Journal of cell biology 2016 📊 467

Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy...

Airyscan Microscopy Confocal Laser Scanning Microscopy Fluorescence Microscopy Multiphoton Microscopy LSM 700 LSM 710 LSM 880 Zeiss LSM 700 Confocal Zeiss LSM 710 Confocal Zeiss LSM 880 Confocal ZEN Alexa Fluor 405 Alexa Fluor 633 DAPI GFP mCherry MitoTracker MitoTracker Deep Red RFP Blocking Cell Culture Cryosectioning Fixation Immunohistochemistry Immunolabeling-enabled Three-Dimensional Imaging of Solvent-Cleared Organs Immunostaining Live Imaging Paraformaldehyde Fixation Permeabilization Primary Culture Transfection Ultramicrotome Whole Mount Mouse Embryonic Fibroblast Zeiss LSM 700 Confocal Zeiss LSM 710 Confocal Zeiss LSM 880 Confocal Volocity ZEN ZEN University of Dundee Abcam Bio-Rad Cell Signaling Technology Merck Roche Santa Cruz Biotechnology Sigma-Aldrich Thermo Fisher Scientific Vector Laboratories Chicken Mouse Rabbit Rat Sheep Zeiss Airyscan Zeiss Plan-Apochromat 20x Zeiss Plan-Neofluar 40x

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⭐ Foundational

A general method to fine-tune fluorophores for live-cell and in vivo imaging.

Nature methods 2017 📊 546

Pushing the frontier of fluorescence microscopy requires the design of enhanced fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered azetidine rings...

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