Technique • 133 papers
"Smart fluorophores", such as reversibly switchable fluorescent proteins, are crucial for advanced fluorescence imaging. However, only a limited number of such labels is available, and...
Abstract Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we...
The two Condensin complexes in human cells are essential for mitotic chromosome structure. We used homozygous genome editing to fluorescently tag Condensin I and II...
Nonlinear structured illumination microscopy has achieved resolutions down to 40 nm and imaging speeds up to 2.86 frames/s. However, relatively few realizations of NSIM have...
We introduce FASER, a software package designed to simulate the excitation point spread functions (PSFs) of microscopes. It is written in Python as a plugin...
In this review, we discuss different super-resolution microscopy (SRM) techniques employed to study viral structures and virus composition with nanometric resolution. We describe the basic...
Abstract Investigating the fine structure of mitochondria and their dynamic interactions with other organelles is crucial for unraveling the mechanisms underlying mitochondrial-related diseases. The development...
ABSTRACT The field of Neurochemistry spent decades trying to understand how the brain works, from nano to macroscale and across diverse species. Technological advancements over...
Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of...
The genomes of metazoans are organized at multiple spatial scales, ranging from the double helix of DNA to whole chromosomes. The intermediate genomic scale of...